RT Generic
T1 Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
A1 Ruiz-Duque, Borja
A1 Banuls, Lucia
A1 Reinoso-Arija, Rocio
A1 Carrasco-Hernandez, Laura
A1 Caballero-Eraso, Candelaria
A1 Dasi, Francisco
A1 Lopez-Campos, Jose Luis
K1 alpha1-antitripsin
K1 serum
K1 plasma
K1 blood concentration
K1 nephelometry
K1 turbidimetry
K1 Alpha-1-antitrypsin deficiency
K1 Alpha(1)-antitrypsin
K1 Diagnosis
AB Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by ELISA (19.8%) and turbidimetry (13.5%). Altogether, 182 (87.9%) cases expressed their results in units of gram, while 16 (7.7%) articles expressed them in units of mole. Only 2.9% articles referred to the standard used, 43.5% articles indicated the commercial kit used, and 36.2% indicated the analyzer used. Conclusions: The technical aspects of these determinations are not always reported in the literature. Journals should be attentive to these technical requirements and ensure that they are included in the works in which AAT is determined in order to ensure a correct interpretation of the study findings.
PB Mdpi
YR 2021
FD 2021-11-01
LK https://hdl.handle.net/10668/27935
UL https://hdl.handle.net/10668/27935
LA en
DS RISalud
RD Apr 12, 2025