RT Journal Article
T1 Transcriptomic Analysis of a Diabetic Skin-Humanized Mouse Model Dissects Molecular Pathways Underlying the Delayed Wound Healing Response.
A1 León, Carlos
A1 García-García, Francisco
A1 Llames, Sara
A1 García-Pérez, Eva
A1 Carretero, Marta
A1 Arriba, María Del Carmen
A1 Dopazo, Joaquín
A1 Del Río, Marcela
A1 Escámez, María José
A1 Martínez-Santamaría, Lucía
K1 diabetes
K1 enrichment analysis
K1 skin-humanized mice
K1 transcriptomics
K1 wound healing
AB Defective healing leading to cutaneous ulcer formation is one of the most feared complications of diabetes due to its consequences on patients' quality of life and on the healthcare system. A more in-depth analysis of the underlying molecular pathophysiology is required to develop effective healing-promoting therapies for those patients. Major architectural and functional differences with human epidermis limit extrapolation of results coming from rodents and other small mammal-healing models. Therefore, the search for reliable humanized models has become mandatory. Previously, we developed a diabetes-induced delayed humanized wound healing model that faithfully recapitulated the major histological features of such skin repair-deficient condition. Herein, we present the results of a transcriptomic and functional enrichment analysis followed by a mechanistic analysis performed in such humanized wound healing model. The deregulation of genes implicated in functions such as angiogenesis, apoptosis, and inflammatory signaling processes were evidenced, confirming published data in diabetic patients that in fact might also underlie some of the histological features previously reported in the delayed skin-humanized healing model. Altogether, these molecular findings support the utility of such preclinical model as a valuable tool to gain insight into the molecular basis of the delayed diabetic healing with potential impact in the translational medicine field.
YR 2020
FD 2020-12-31
LK http://hdl.handle.net/10668/16909
UL http://hdl.handle.net/10668/16909
LA en
DS RISalud
RD Apr 11, 2025