RT Journal Article
T1 Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering
A1 Sanchez-Quevedo, M.C.
A1 Alaminos, M.
A1 Capitan, L.M.
A1 Moreu, G.
A1 Garzon, I.
A1 Crespo, P.V.
A1 Campos, A
K1 Técnicas de Cultivo de Célula
K1 Supervivencia Celular
K1 Células Cultivadas
K1 Microanálisis por Sonda Electrónica
K1 Fibrina
K1 Fibroblastos
K1 Expresión Génica
K1 Perfilación de la Expresión Génica
K1 Humanos
K1 Hidrogeles
K1 Inmunohistoquímica
K1 Queratinocitos
K1 Microscopía Electrónica
K1 Mucosa Bucal
K1 Análisis de Secuencia en Orden de Oligonucleótido
K1 Sefarosa
K1 Ingeniería de Tejidos
K1 Fibrin-agarose
K1 Constructs
K1 Tissue engineering
AB Reconstruction of large oral mucosa defects is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show several similarities with native human oral mucosa.
PB Universidad de Murcia
SN 0213-3911
YR 2007
FD 2007-06
LK http://hdl.handle.net/10668/683
UL http://hdl.handle.net/10668/683
LA en
NO Sanchez-Quevedo MC, Alaminos M, Capitan LM, Moreu G, Garzon I et al.Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007 Jun;22(6):631-40
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 17, 2025