RT Journal Article
T1 Detection of glycidic receptors in microalgae using glycodendrons as probes: a new tool for studies on cell surface interactions
A1 Goncalves, Cintia de Almeida
A1 Figueiredo, Rute Cunha
A1 Giani, Alessandra
A1 Collado, Daniel
A1 Perez-Inestrosa, Ezequiel
A1 Rojo, Javier
A1 Figueredo, Cleber Cunha
K1 Cell interaction
K1 Lectins
K1 Carbohydrates
K1 Algae
K1 Fluorescent probes
K1 Cell recognition
K1 Dc-sign
K1 Lectin-binding
K1 Mannose-binding
K1 Contact inhibition
K1 Trans-infection
K1 Green-algae
K1 Recognition
K1 Protein
K1 Hemagglutination
K1 Proliferation
AB Cell recognition, adhesion, and internalization are involved in infectious, reproductive, and inflammatory processes and are generally mediated by interactions between molecules located in the cell membrane and the extracellular matrix. These processes can decrease proliferation rates and they are well known for bacteria, fungi, and animals, but there is a lack of knowledge regarding autotrophic cells. Carbohydrates and proteins (e.g., lectins) are important molecules for cell interactions and information about these molecules is essential to better understand many biological phenomena in uni- or multicellular organisms. Most studies focus on the identification of the carbohydrates present on the cell surface by using labeled lectins. Alternatively, here we present a pioneer research performed by using three different labeled carbohydrates in a multivalent presentation (glycodendrons) to detect the presence of carbohydrate receptors (e.g., lectins) on cell surfaces of 12 algal species. The goal of this study was to detect some specificity in these molecular interactions, but in a reverse way in comparison to that commonly described in the literature. We tested trivalent molecules containing residuals of D-mannose, L-fucose, or N-acetyl-galactosamine to identify their bindings with the corresponding lectins expressed on cell surfaces. We envisage that our new approach could be an alternative tool for taxonomic and physiological studies on microalgae or even on other groups of organisms. Based on our results, the receptors found in the cell surface of the algal species tend to differ in composition, quantity, and distribution. The differences were mainly species-specific, since no patterns were identified at higher taxonomic level. Moreover, like lectins, labeled carbohydrates were proved to be a reliable tool for the study of cell surface composition.
PB Springer
SN 0921-8971
YR 2019
FD 2019-02-01
LK http://hdl.handle.net/10668/18641
UL http://hdl.handle.net/10668/18641
LA en
DS RISalud
RD Apr 8, 2025