RT Journal Article
T1 Epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole for six Candida species as determined by the colorimetric Sensititre YeastOne method.
A1 Cantón, Emilia
A1 Pemán, Javier
A1 Iñiguez, Carmen
A1 Hervás, David
A1 Lopez-Hontangas, Jose L
A1 Pina-Vaz, Cidalia
A1 Camarena, Juan J
A1 Campos-Herrero, Isolina
A1 García-García, Inmaculada
A1 García-Tapia, Ana M
A1 Guna, Remedios
A1 Merino, Paloma
A1 Pérez del Molino, Luisa
A1 Rubio, Carmen
A1 Suárez, Anabel
K1 Azoles
K1 Antifúngicos
K1 Candidiasis
K1 Pruebas de sensibilidad microbiana
AB In the absence of clinical breakpoints (CBP), epidemiological cutoff values (ECVs) are useful to separate wild-type (WT) isolates (without mechanisms of resistance) from non-WT isolates (those that can harbor some resistance mechanisms), which is the goal of susceptibility tests. Sensititre YeastOne (SYO) is a widely used method to determine susceptibility of Candida spp. to antifungal agents. The CLSI CBP have been established, but not for the SYO method. The ECVs for four azoles, obtained using MIC distributions determined by the SYO method, were calculated via five methods (three statistical methods and based on the MIC50 and modal MIC). Respectively, the median ECVs (in mg/liter) of the five methods for fluconazole, itraconazole, posaconazole, and voriconazole (in parentheses: the percentage of isolates inhibited by MICs equal to or less than the ECVs; the number of isolates tested) were as follows: 2 (94.4%; 944), 0.5 (96.7%; 942), 0.25 (97.6%; 673), and 0.06 (96.7%; 849) for Candida albicans; 4 (86.1%; 642), 0.5 (99.4%; 642), 0.12 (93.9%; 392), and 0.06 (86.9%; 559) for C. parapsilosis; 8 (94.9%; 175), 1 (93.7%; 175), 2 (93.6%; 125), and 0.25 (90.4%; 167) for C. tropicalis; 128 (98.6%; 212), 4 (95.8%; 212), 4 (96.0%; 173), and 2 (98.5; 205) for C. glabrata; 256 (100%; 53), 1 (98.1%; 53), 1 (100%; 33), and 1 (97.9%; 48) for C. krusei; 4 (89.2%; 93), 0.5 (100%; 93), 0.25 (100%; 33), and 0.06 (87.7%; 73) for C. orthopsilosis. All methods included ≥94% of isolates and yielded similar ECVs (within 1 dilution). These ECVs would be suitable for monitoring emergence of isolates with reduced susceptibility by using the SYO method.
PB American Society for Microbiology
SN 0095-1137
YR 2013
FD 2013-08
LK http://hdl.handle.net/10668/2362
UL http://hdl.handle.net/10668/2362
LA en
NO Cantón E, Pemán J, Iñiguez C, Hervás D, Lopez-Hontangas JL, Pina-Vaz C, et al. Epidemiological cutoff values for fluconazole, itraconazole, posaconazole, and voriconazole for six Candida species as determined by the colorimetric Sensititre YeastOne method. J Clin Microbiol. 2013 Aug; 51(8):2691-5
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 7, 2025