RT Journal Article
T1 Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets.
A1 Hassouneh, Fakhri
A1 Lopez-Sejas, Nelson
A1 Campos, Carmen
A1 Sanchez-Correa, Beatriz
A1 Tarazona, Raquel
A1 Pera, Alejandra
A1 Solana, Rafael
K1 CD57
K1 Eomes
K1 T-bet
K1 T-cells
K1 Ageing
K1 Cytomegalovirus (CMV)
AB The differential impact of ageing and cytomegalovirus (CMV) latent infection on human T-cell subsets remains to some extent controversial. The purpose of this study was to analyse the expression of the transcription factors T-bet and Eomes and CD57 on CD4+, CD4hiCD8lo and CD8+ T-cell subsets in healthy individuals, stratified by age and CMV serostatus. The percentage of CD4+ T-cells expressing T-bet or Eomes was very low, in particular in CD4+ T-cells from young CMV-seronegative individuals, and were higher in CMV-seropositive older individuals, in both CD57- and CD57+ CD4+ T-cells. The study of the minor peripheral blood double-positive CD4hiCD8lo T-cells showed that the percentage of these T-cells expressing both Eomes and T-bet was higher compared to CD4+ T-cells. The percentage of CD4hiCD8lo T-cells expressing T-bet was also associated with CMV seropositivity and the coexpression of Eomes, T-bet and CD57 on CD4hiCD8lo T-cells was only observed in CMV-seropositive donors, supporting the hypothesis that these cells are mature effector memory cells. The percentage of T-cells expressing Eomes and T-bet was higher in CD8+ T-cells than in CD4+ T-cells. The percentages of CD8+ T-cells expressing Eomes and T-bet increased with age in CMV-seronegative and -seropositive individuals and the percentages of CD57- CD8+ and CD57+ CD8+ T-cells coexpressing both transcription factors were similar in the different groups studied. These results support that CMV chronic infection and/or ageing are associated to the expansion of highly differentiated CD4+, CD4hiCD8lo and CD8+ T-cells that differentially express T-bet and Eomes suggesting that the expression of these transcription factors is essential for the generation and development of an effector-memory and effector T lymphocytes involved in conferring protection against chronic CMV infection.
PB MDPI
YR 2017
FD 2017-06-26
LK http://hdl.handle.net/10668/11359
UL http://hdl.handle.net/10668/11359
LA en
NO Hassouneh F, Lopez-Sejas N, Campos C, Sanchez-Correa B, Tarazona R, Pera A, et al. Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets. Int J Mol Sci. 2017 Jun 29;18(7):1391
NO This work was supported by grants PI13/02691 and PI16/01615 (to Rafael Solana) from Instituto de Salud Carlos III, Spanish Ministry of Health, SAF2013-46161-R (to Raquel Tarazona) from the Ministry of Science and Innovation of Spain and GR15183 from Junta de Extremadura (to Raquel Tarazona). These grants were co-financed by the European Regional Development Fund (FEDER).
DS RISalud
RD Apr 12, 2025