RT Journal Article
T1 Synergistic Activity of Niclosamide in Combination With Colistin Against Colistin-Susceptible and Colistin-Resistant Acinetobacter baumannii and Klebsiella pneumoniae.
A1 Ayerbe-Algaba, Rafael
A1 Gil-Marqués, María Luisa
A1 Jiménez-Mejías, Manuel Enrique
A1 Sánchez-Encinales, Viviana
A1 Parra-Millán, Raquel
A1 Pachón-Ibáñez, María Eugenia
A1 Pachón, Jerónimo
A1 Smani, Younes
K1 Acinetobacter baumannii
K1 Klebsiella pneumoniae
K1 colistin
K1 niclosamide
K1 repurposing drug
K1 synergistic effect
AB Colistin is among the few antibiotics effective against multidrug-resistant Acinetobacter baumannii and Klebsiella pneumoniae clinical isolates. However, in the last few years, colistin-resistant A. baumannii and K. pneumoniae strains have emerged. Therefore, combination therapies, between colistin and other old drugs, restoring the activity of colistin are required. The main objective of this study was to analyse the activity of niclosamide, an anthelmintic drug, in combination with colistin against colistin-susceptible (Col-S) and colistin-resistant (Col-R) A. baumannii and K. pneumoniae. The MIC were determined by microdilution assay and the time-kill curves were performed. The zeta potential of Col-S and Col-R of A. baumannii and K. pneumoniae in presence of niclosamide was assessed. Niclosamide in combination with colistin showed improved activity against Col-S and Col-R A. baumannii and K. pneumoniae. Time-killing curves showed synergic activity between niclosamide and colistin against Col-S and Col-R A. baumannii and K. pneumoniae, especially when niclosamide or colistin was added for second time at 4 h of the 24 h killing curve. Col-R A. baumannii and K. pneumoniae in presence of niclosamide exhibited a greater negative charge (-34.95 ± 0.35 mV and -38.85 ± 0.92 mV; P
YR 2018
FD 2018-10-03
LK http://hdl.handle.net/10668/13106
UL http://hdl.handle.net/10668/13106
LA en
DS RISalud
RD Apr 5, 2025