RT Journal Article
T1 Lysozyme crystallization in hydrogel media under ultrasound irradiation.
A1 Savchenko, Mariia
A1 Hurtado, Manuel
A1 Lopez-Lopez, Modesto T
A1 Rus, Guillermo
A1 Alvarez de Cienfuegos, Luis
A1 Melchor, Juan
A1 Gavira, Jose A
K1 Hydrogels
K1 Lysozyme
K1 Nucleation
K1 Protein crystallization
K1 Ultrasound
AB Sonocrystallization implies the application of ultrasound radiation to control the nucleation and crystal growth depending on the actuation time and intensity. Its application allows to induce nucleation at lower supersaturations than required under standard conditions. Although extended in inorganic and organic crystallization, it has been scarcely explored in protein crystallization. Now, that industrial protein crystallization is gaining momentum, the interest on new ways to control protein nucleation and crystal growth is advancing. In this work we present the development of a novel ultrasound bioreactor to study its influence on protein crystallization in agarose gel. Gel media minimize convention currents and sedimentation, favoring a more homogeneous and stable conditions to study the effect of an externally generated low energy ultrasonic irradiation on protein crystallization avoiding other undesired effects such as temperature increase, introduction of surfaces which induce nucleation, destructive cavitation phenomena, etc. In-depth statistical analysis of the results has shown that the impact of ultrasound in gel media on crystal size populations are statistically significant and reproducible.
PB Elsevier BV
YR 2022
FD 2022-07-14
LK http://hdl.handle.net/10668/22538
UL http://hdl.handle.net/10668/22538
LA en
NO Savchenko M, Hurtado M, Lopez-Lopez MT, Rus G, Álvarez de Cienfuegos L, Melchor J, et al. Lysozyme crystallization in hydrogel media under ultrasound irradiation. Ultrason Sonochem. 2022 Aug;88:106096.
DS RISalud
RD Apr 8, 2025