RT Journal Article
T1 Gut Microbiota Composition in Male Rat Models under Different Nutritional Status and Physical Activity and Its Association with Serum Leptin and Ghrelin Levels.
A1 Queipo-Ortuño, María Isabel
A1 Seoane, Luisa María
A1 Murri, Mora
A1 Pardo, María
A1 Gómez-Zumaquero, Juan Miguel
A1 Cardona, Fernando
A1 Casanueva, Felipe
A1 Tinahones, Francisco J
K1 Modelos Animales
K1 Leptina
K1 Apetito
K1 Electroforesis en Gel de Gradiente Desnaturalizante
K1 Ejercicio
K1 Metabolismo
K1 Estado nutricional
AB BACKGROUNDSeveral evidences indicate that gut microbiota is involved in the control of host energy metabolism.OBJECTIVETo evaluate the differences in the composition of gut microbiota in rat models under different nutritional status and physical activity and to identify their associations with serum leptin and ghrelin levels.METHODSIN A CASE CONTROL STUDY, FORTY MALE RATS WERE RANDOMLY ASSIGNED TO ONE OF THESE FOUR EXPERIMENTAL GROUPS: ABA group with food restriction and free access to exercise; control ABA group with food restriction and no access to exercise; exercise group with free access to exercise and feed ad libitum and ad libitum group without access to exercise and feed ad libitum. The fecal bacteria composition was investigated by PCR-denaturing gradient gel electrophoresis and real-time qPCR.RESULTSIn restricted eaters, we have found a significant increase in the number of Proteobacteria, Bacteroides, Clostridium, Enterococcus, Prevotella and M. smithii and a significant decrease in the quantities of Actinobacteria, Firmicutes, Bacteroidetes, B. coccoides-E. rectale group, Lactobacillus and Bifidobacterium with respect to unrestricted eaters. Moreover, a significant increase in the number of Lactobacillus, Bifidobacterium and B. coccoides-E. rectale group was observed in exercise group with respect to the rest of groups. We also found a significant positive correlation between the quantity of Bifidobacterium and Lactobacillus and serum leptin levels, and a significant and negative correlation among the number of Clostridium, Bacteroides and Prevotella and serum leptin levels in all experimental groups. Furthermore, serum ghrelin levels were negatively correlated with the quantity of Bifidobacterium, Lactobacillus and B. coccoides-Eubacterium rectale group and positively correlated with the number of Bacteroides and Prevotella.CONCLUSIONSNutritional status and physical activity alter gut microbiota composition affecting the diversity and similarity. This study highlights the associations between gut microbiota and appetite-regulating hormones that may be important in terms of satiety and host metabolism.
PB Public Library of Science
YR 2013
FD 2013-05
LK http://hdl.handle.net/10668/1145
UL http://hdl.handle.net/10668/1145
LA en
NO Queipo-Ortuño MI, Seoane LM, Murri M, Pardo M, Gomez-Zumaquero JM, Cardona F, et al. Gut Microbiota Composition in Male Rat Models under Different Nutritional Status and Physical Activity and Its Association with Serum Leptin and Ghrelin Levels. PLoS ONE. 2013; 8(5):e65465
NO Journal Article; The research group belongs to the “Centros de Investigación en Red” (CIBEROBN, CB06/03/0018) of the “Instituto de Salud Carlos III.
DS RISalud
RD Apr 20, 2025