%0 Journal Article
%A López-Saavedra, Ana
%A Gómez-Cabello, Daniel
%A Domínguez-Sánchez, María Salud
%A Mejías-Navarro, Fernando
%A Fernández-Ávila, María Jesús
%A Dinant, Christoffel
%A Martínez-Macías, María Isabel
%A Bartek, Jiri
%A Huertas, Pablo
%T A genome-wide screening uncovers the role of CCAR2 as an antagonist of DNA end resection.
%D 2016
%U http://hdl.handle.net/10668/10347
%X There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these pathways; in this study we took advantage of the SeeSaw Reporter, in which the repair of double-strand breaks by homology-independent or -dependent mechanisms is distinguished by the accumulation of green or red fluorescence, respectively. Using a genome-wide human esiRNA (endoribonuclease-prepared siRNA) library, we isolate genes that control the recombination/end-joining ratio. Here we report that two distinct sets of genes are involved in the control of the balance between NHEJ and HR: those that are required to facilitate recombination and those that favour NHEJ. This last category includes CCAR2/DBC1, which we show inhibits recombination by limiting the initiation and the extent of DNA end resection, thereby acting as an antagonist of CtIP.
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