RT Journal Article
T1 Cylindrospermopsin-Microcystin-LR Combinations May Induce Genotoxic and Histopathological Damage in Rats
A1 Díez-Quijada, Leticia
A1 Medrano-Padial, Concepción
A1 Llana-Ruiz-Cabello, María
A1 Catunescu, Giorgiana M.
A1 Moyano, Rosario
A1 Risalde, Maria A.
A1 Cameán, Ana M.
A1 Jos, Ángeles
K1 In vivo
K1 Genotoxicity
K1 Cylindrospermopsin
K1 Microcystin-LR
K1 Micronucleus
K1 Comet assay
K1 Enzyme-modified comet assay
K1 Rats
K1 Genotoxicidad
K1 Cianotoxinas
K1 Pruebas de micronúcleos
K1 Ensayo cometa
K1 Ratas
AB Cylindrospermopsin (CYN) and microcystins (MC) are cyanotoxins that can occur simultaneously in contaminated water and food. CYN/MC-LR mixtures previously investigated in vitro showed an induction of micronucleus (MN) formation only in the presence of the metabolic fraction S9. When this is the case, the European Food Safety Authority recommends a follow up to in vivo testing. Thus, rats were orally exposed to 7.5 + 75, 23.7 + 237, and 75 + 750 μg CYN/MC-LR/kg body weight (b.w.). The MN test in bone marrow was performed, and the standard and modified comet assays were carried out to measure DNA strand breaks or oxidative DNA damage in stomach, liver, and blood cells. The results revealed an increase in MN formation in bone marrow, at all the assayed doses. However, no DNA strand breaks nor oxidative DNA damage were induced, as shown in the comet assays. The histopathological study indicated alterations only in the highest dose group. Liver was the target organ showing fatty degeneration and necrotic hepatocytes in centrilobular areas, as well as a light mononuclear inflammatory periportal infiltrate. Additionally, the stomach had flaking epithelium and mild necrosis of epithelial cells. Therefore, the combined exposure to cyanotoxins may induce genotoxic and histopathological damage in vivo.
PB MDPI
YR 2020
FD 2020-05-26
LK http://hdl.handle.net/10668/3743
UL http://hdl.handle.net/10668/3743
LA en
NO Díez-Quijada L, Medrano-Padial C, Llana-Ruiz-Cabello M, Cătunescu GM, Moyano R, Risalde MA, et al. Cylindrospermopsin-Microcystin-LR Combinations May Induce Genotoxic and Histopathological Damage in Rats. Toxins. 2020 May 26;12(6):348
DS RISalud
RD Apr 6, 2025