RT Journal Article
T1 Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium
A1 Pontel, Lucas B.
A1 Langenick, Judith
A1 Rosado, Ivan V.
A1 Zhang, Xiao-Yin
A1 Traynor, David
A1 Kay, Robert R.
A1 Patel, Ketan J.
K1 Xpf
K1 Phagocytosis
K1 Mutagenesis
K1 Dictyostelium
K1 DNA repair
K1 Dna-damage
K1 Discoideum
K1 Bacteria
K1 Resistance
K1 Repair
K1 Methanol
K1 Pathway
K1 Growth
K1 Genes
K1 Ameba
AB As time passes, mutations accumulate in the genomes of all living organisms. These changes promote genetic diversity, but also precipitate ageing and the initiation of cancer. Food is a common source of mutagens, but little is known about how nutritional factors cause lasting genetic changes in the consuming organism. Here, we describe an unusual genetic interaction between DNA repair in the unicellular amoeba Dictyostelium discoideum and its natural bacterial food source. We found that Dictyostelium deficient in the DNA repair nuclease Xpf (xpf-) display a severe and specific growth defect when feeding on bacteria. Despite being proficient in the phagocytosis and digestion of bacteria, over time, xpf(-) Dictyostelium feeding on bacteria cease to grow and in many instances die. The Xpf nuclease activity is required for sustained growth using a bacterial food source. Furthermore, the ingestion of this food source leads to a striking accumulation of mutations in the genome of xpf(-) Dictyostelium. This work therefore establishes Dictyostelium as a model genetic system to dissect nutritional genotoxicity, providing insight into how phagocytosis can induce mutagenesis and compromise survival fitness.
PB Company of biologists ltd
SN 0021-9533
YR 2016
FD 2016-12-15
LK http://hdl.handle.net/10668/19141
UL http://hdl.handle.net/10668/19141
LA en
DS RISalud
RD Apr 7, 2025