RT Journal Article
T1 First identification of blaNDM-1 carbapenemase in blaOXA-94-producing Acinetobacter baumannii ST85 in Spain.
A1 Fernández-Cuenca, Felipe
A1 Pérez-Palacios, Patricia
A1 Galán-Sánchez, Fátima
A1 López-Cerero, Lorena
A1 López-Hernández, Inmaculada
A1 López Rojas, Rafael
A1 Arca-Suárez, Jorge
A1 Díaz-de Alba, Paula
A1 Rodríguez Iglesias, Manuel
A1 Pascual, Alvaro
K1 A. baumannii
K1 NDM-1
K1 OXA-94
K1 ST85
AB NDM-1 carbapenemase is spreading rapidly all over the world, but this metallo-beta-lactamase has just been detected for the first time in an Acinetobacter baumannii (Ab) isolate of the ST85 clone in Spain. The aim of this study was to characterize a NDM-1-producing carbapenem-resistant A. baumannii (CR-Ab) isolate submitted to the Andalusian PIRASOA [infection prevention program] referral laboratory. Carbapenemases were detected by PCR and Sanger DNA sequencing. Whole genome sequencing was performed by NGS (Miseq, Illumina). Resistance genes were identified with RESfinder, while MLSTfinder was used for sequence typing (ST). The genetic location of blaNDM-1 was determined by nuclease S-1/PFGE/hybridization with specific probe. The isolate was susceptible to amikacin and tigecycline and belonged to the ST85 clone. blaOXA-94 and blaNDM-1 were identified by PCR and Sanger DNA sequencing, respectively. The resistance genes aadB, blaADC-25, blaNDM-1, blaOXA-94, msr(E), mph(E) and floR,sul2 were identified by NGS. The chromosome of the isolate contained a defective Tn125 transposon with blaNDM-1 flanked by the insertion sequences ISAbA125 and ISAba14. The blaNDM-1 gene was only detected in the chromosomal DNA. This is the first time that blaNDM-1 has been detected and characterized in a blaOXA-94-producing CR-Ab isolate belonging to the ST85 clone in Spain.
YR 2019
FD 2019-05-04
LK http://hdl.handle.net/10668/13918
UL http://hdl.handle.net/10668/13918
LA en
LA es
DS RISalud
RD Apr 5, 2025