RT Journal Article
T1 Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data
A1 Perea, Cristina
A1 Vázquez-Ágredos, Ana
A1 Ruiz-Leyva, Leandro
A1 Morón, Ignacio
A1 Zúñiga, Jesús Martín
A1 Cendán, Cruz Miguel
K1 Caloric restriction
K1 Grouped mice
K1 Littermate mice
K1 Adrenocorticotropic
K1 Eating behavior
K1 Social behavior
K1 Aging
K1 Oxidative stress
K1 Anxiety
K1 Restricción calórica
K1 Hormona adrenocorticotrópica
K1 Conducta alimentaria
K1 Conducta social
K1 Envejecimiento
K1 Estrés oxidativo
K1 Ansiedad
AB Much of the research done on aging, oxidative stress, anxiety, and cognitive and social behavior in rodents has focused on caloric restriction (CR). This often involves several days of single housing, which can cause numerous logistical problems, as well as cognitive and social dysfunctions. Previous results in our laboratory showed the viability of long-term CR in grouped rats. Our research has studied the possibility of CR in grouped female and male littermates and unrelated CB6F1/J (C57BL/6J × BALBc/J hybrid strain) mice, measuring: (i) possible differences in body mass proportions between mice in ad libitum and CR conditions (at 70% of ad libitum), (ii) aggressive behavior, using the number of pushes and chasing behavior time as an indicator and social behavior using the time under the feeder as indicator, and (iii) difference in serum adrenocorticotropic hormone (ACTH) concentrations (stress biomarker), under ad libitum and CR conditions. Results showed the impossibility of implementing CR in unrelated male mice. In all other groups, CR was possible, with a less aggressive behavior (measured only with the number of pushes) observed in the unrelated female mice under CR conditions. In that sense, the ACTH levels measured on the last day of CR showed no difference in stress levels. These results indicate that implementantion of long-term CR in mice can be optimized technically and also related to their well-being by grouping animals, in particular, related mice.
PB Frontiers
YR 2021
FD 2021-04-15
LK http://hdl.handle.net/10668/4383
UL http://hdl.handle.net/10668/4383
LA en
NO Perea C, Vázquez-Ágredos A, Ruiz-Leyva L, Morón I, Zúñiga JM, Cendán CM. Caloric Restriction in Group-Housed Mice: Littermate and Sex Influence on Behavioral and Hormonal Data. Front Vet Sci. 2021 Apr 15;8:639187
DS RISalud
RD Apr 7, 2025