RT Journal Article
T1 Combinatorial effects of splice variants modulate function of Aiolos
A1 Caballero, Rosalia
A1 Setien, Fernando
A1 Lopez-Serra, Lidia
A1 Boix-Chornet, Manuel
A1 Fraga, Mario F
A1 Ropero, Santiago
A1 Megias, Diego
A1 Alaminos, Miguel
A1 Sanchez-Tapia, Eva M
A1 Montoya, Maria C
A1 Esteller, Manel
A1 Gonzalez-Sarmiento, Rogelio
A1 Ballestar, Esteban
K1 Empalme Alternativo
K1 Animales
K1 Linfocitos B
K1 Línea Celular
K1 Línea Celular Tumoral
K1 Dimerización
K1 Regulación de la Expresión Génica
K1 Histona Desacetilasas
K1 Histonas
K1 Humanos
K1 Factor de Transcripción Ikaros
K1 Isoformas de Proteínas
K1 Factores de Transcripción
K1 Dedos de Zinc
K1 Aiolos
K1 Chromatin
K1 Epigenetics
K1 Ikaros
K1 Isoforms
K1 Mi-2/NuRD
AB The transcription factor Aiolos (also known as IKZF3), a member of the Ikaros family of zinc-finger proteins, plays an important role in the control of B lymphocyte differentiation and proliferation. Previously, multiple isoforms of Ikaros family members arising from differential splicing have been described and we now report a number of novel isoforms of Aiolos. It has been demonstrated that full-length Ikaros family isoforms localize to heterochromatin and that they can associate with complexes containing histone deacetylase (HDAC). In this study, for the first time we directly investigate the cellular localization of various Aiolos isoforms, their ability to heterodimerize with Ikaros and associate with HDAC-containing complexes, and the effects on histone modification and binding to putative targets. Our work demonstrates that the cellular activities of Aiolos isoforms are dependent on combinations of various functional domains arising from the differential splicing of mRNA transcripts. These data support the general principle that the function of an individual protein is modulated through alternative splicing, and highlight a number of potential implications for Aiolos in normal and aberrant lymphocyte function.
PB COMPANY OF BIOLOGISTS LTD
SN 0021-9533
YR 2007
FD 2007-08-01
LK http://hdl.handle.net/10668/612
UL http://hdl.handle.net/10668/612
LA en
NO Caballero R, Setien F, Lopez-Serra L, Boix-Chornet M, Fraga MF, Ropero S, et al. Combinatorial effects of splice variants modulate function of Aiolos. J. Cell. Sci.. 2007 Aug; 120(Pt 15):2619-30
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 11, 2025