RT Journal Article
T1 SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
A1 García-León, Juan Antonio
A1 Kumar, Manoj
A1 Boon, Ruben
A1 Chau, David
A1 One, Jennifer
A1 Wolfs, Esther
A1 Eggermont, Kristel
A1 Berckmans, Pieter
A1 Gunhanlar, Nilhan
A1 de Vrij, Femke
A1 Lendemeijer, Bas
A1 Pavie, Benjamin
A1 Corthout, Nikky
A1 Kushner, Steven A
A1 Dávila, José Carlos
A1 Lambrichts, Ivo
A1 Hu, Wei-Shou
A1 Verfaillie, Catherine M
K1 amyotrophic lateral sclerosis
K1 disease modeling
K1 drug screening
K1 induced pluripotent stem cells (iPSCs)
K1 multiple sclerosis
K1 myelination
K1 oligodendrocyte
AB Scarce access to primary samples and lack of efficient protocols to generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding of OL biology and the development of novel therapies. Here, we demonstrate that overexpression of the transcription factor SOX10 is sufficient to generate surface antigen O4-positive (O4+) and myelin basic protein-positive OLs from hPSCs in only 22 days, including from patients with multiple sclerosis or amyotrophic lateral sclerosis. The SOX10-induced O4+ population resembles primary human OLs at the transcriptome level and can myelinate neurons in vivo. Using in vitro OL-neuron co-cultures, myelination of neurons by OLs can also be demonstrated, which can be adapted to a high-throughput screening format to test the response of pro-myelinating drugs. In conclusion, we provide an approach to generate OLs in a very rapid and efficient manner, which can be used for disease modeling, drug discovery efforts, and potentially for therapeutic OL transplantation.
YR 2018
FD 2018-01-11
LK http://hdl.handle.net/10668/12017
UL http://hdl.handle.net/10668/12017
LA en
DS RISalud
RD Apr 12, 2025