RT Journal Article
T1 MicroRNA Profile of HCV Spontaneous Clarified Individuals, Denotes Previous HCV Infection.
A1 Brochado-Kith, Óscar
A1 Gómez Sanz, Alicia
A1 Real, Luis Miguel
A1 Crespo García, Javier
A1 Ryan Murúa, Pablo
A1 Macías, Juan
A1 Cabezas González, Joaquín
A1 Troya, Jesús
A1 Pineda, Juan Antonio
A1 Arias Loste, María Teresa
A1 Díez Viñas, Victorino
A1 Jiménez-Sousa, María Ángeles
A1 Medrano de Dios, Luz María
A1 Cuesta De la Plaza, Isabel
A1 Monzón Fernández, Sara
A1 Resino García, Salvador
A1 Fernández-Rodríguez, Amanda
K1 MicroRNAs
K1 chronic hepatitis C
K1 lipid metabolism
K1 spontaneous HCV clarification
AB Factors involved in the spontaneous cleareance of a hepatitis C (HCV) infection are related to both HCV and the interaction with the host immune system, but little is known about the consequences after a spontaneous resolution. The main HCV extrahepatic reservoir is the peripheral blood mononuclear cells (PBMCs), and their transcriptional profile provides us information of innate and adaptive immune responses against an HCV infection. MicroRNAs regulate the innate and adaptive immune responses, and they are actively involved in the HCV cycle. High Throughput sequencing was used to analyze the miRNA profiles from PBMCs of HCV chronic naïve patients (CHC), individuals that spontaneously clarified HCV (SC), and healthy controls (HC). We did not find any differentially expressed miRNAs between SC and CHC. However, both groups showed similar expression differences (21 miRNAs) with respect to HC. This miRNA signature correctly classifies HCV-exposed (CHC and SC) vs. HC, with the has-miR-21-3p showing the best performance. The potentially targeted molecular pathways by these 21 miRNAs mainly belong to fatty acids pathways, although hippo signaling, extracellular matrix (ECM) interaction, proteoglycans-related, and steroid biosynthesis pathways were also altered. These miRNAs target host genes involved in an HCV infection. Thus, an HCV infection promotes molecular alterations in PBMCs that can be detected after an HCV spontaneous resolution, and the 21-miRNA signature is able to identify HCV-exposed patients (either CHC or SC).
SN 2077-0383
YR 2019
FD 2019-06-14
LK http://hdl.handle.net/10668/14129
UL http://hdl.handle.net/10668/14129
LA en
DS RISalud
RD Apr 20, 2025