%0 Journal Article
%A Peinado, Paola
%A Andrades, Alvaro
%A Cuadros, Marta
%A Rodriguez, Maria Isabel
%A Coira, Isabel F
%A Garcia, Daniel J
%A Álvarez-Perez, Juan Carlos
%A Baliñas-Gavira, Carlos
%A Arenas, Alberto M
%A Patiño-Mercau, Juan Rodrigo
%A Sanjuan-Hidalgo, Juan
%A Romero, Octavio A
%A Montuenga, Luis M
%A Carretero, Julian
%A Sanchez-Cespedes, Montserrat
%A Medina, Pedro P
%T Comprehensive Analysis of SWI/SNF Inactivation in Lung Adenocarcinoma Cell Models.
%D 2020
%@ 2072-6694
%U https://hdl.handle.net/10668/28417
%X Mammalian SWI/SNF (SWitch/Sucrose Non-Fermentable) complexes are ATP-dependent chromatin remodelers whose subunits have emerged among the most frequently mutated genes in cancer. Studying SWI/SNF function in cancer cell line models has unveiled vulnerabilities in SWI/SNF-mutant tumors that can lead to the discovery of new therapeutic drugs. However, choosing an appropriate cancer cell line model for SWI/SNF functional studies can be challenging because SWI/SNF subunits are frequently altered in cancer by various mechanisms, including genetic alterations and post-transcriptional mechanisms. In this work, we combined genomic, transcriptomic, and proteomic approaches to study the mutational status and the expression levels of the SWI/SNF subunits in a panel of 38 lung adenocarcinoma (LUAD) cell lines. We found that the SWI/SNF complex was mutated in more than 76% of our LUAD cell lines and there was a high variability in the expression of the different SWI/SNF subunits. These results underline the importance of the SWI/SNF complex as a tumor suppressor in LUAD and the difficulties in defining altered and unaltered cell models for the SWI/SNF complex. These findings will assist researchers in choosing the most suitable cellular models for their studies of SWI/SNF to bring all of its potential to the development of novel therapeutic applications.
%K SWI/SNF complex
%K cell models
%K epigenetics
%K lung adenocarcinoma
%K lung cancer
%K multi-omics
%~