RT Journal Article
T1 In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute.
A1 Carriel, Víctor
A1 Scionti, Giuseppe
A1 Campos, Fernando
A1 Roda, Olga
A1 Castro, Begoña
A1 Cornelissen, Maria
A1 Garzon, Ingrid
A1 Alaminos, Miguel
K1 adipose-derived mesenchymal stem cells
K1 biomimetic nerve substitute
K1 cell-biomaterial interactions
K1 extracellular matrix
K1 fibrin-agarose hydrogels
K1 nanostructured biomaterials
K1 neural tissue engineering
AB Neural tissue engineering is focused on the design of novel biocompatible substitutes to repair peripheral nerve injuries. In this paper we describe a nanostructured fibrin-agarose bioartificial nerve substitute (NFABNS), based on nanostructured fibrin-agarose hydrogels (FAHs) with human adipose-derived mesenchymal stem cells (HADMSCs). These NFABNSs were mechanically characterized and HADMSCs behaviour was evaluated using histological and ultrastructural techniques. Mechanical characterization showed that the NFABNSs were resistant, flexible and elastic, with a high deformation capability. Histological analyses carried out in vitro during 16 days revealed that the number of HADMSCs decreased over time, with a significant increase after 16 days. HADMSCs formed cell clusters and degraded the surrounding scaffold during this time; additionally, HADMSCs showed active cell proliferation and cytoskeletal remodelling, with a progressive synthesis of extracellular matrix molecules. Finally, this study demonstrated that it is possible to generate biologically active and mechanically stable tissue-like substitutes with specific dimensions, based on the use of HADMSCs, FAHs and a nanostructure technique. However, in vivo analyses are needed to demonstrate their potential usefulness in peripheral nerve repair. Copyright © 2015 John Wiley & Sons, Ltd.
PB Hindawi Limited
YR 2015
FD 2015-04-29
LK http://hdl.handle.net/10668/9972
UL http://hdl.handle.net/10668/9972
LA en
NO Carriel V, Scionti G, Campos F, Roda O, Castro B, Cornelissen M, et al. In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute. J Tissue Eng Regen Med. 2017 May;11(5):1412-1426.
DS RISalud
RD Apr 15, 2025