RT Journal Article
T1 CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets.
A1 Medrano, Mayte
A1 Lemus-Conejo, Ana
A1 Lopez, Sergio
A1 Millan-Linares, Maria C
A1 Rosillo, Maria A
A1 Muñiz, Manuel
A1 Calderon, Rocio
A1 Jaramillo, Sara
A1 Perez-Simon, Jose A
A1 Muriana, Francisco J G
A1 Abia, Rocio
K1 Dietary fatty acids
K1 Lymphocyte subsets
K1 Obesity
K1 Oleic acid
K1 Palmitic acid
AB Obesity is associated with disruptions in the adaptive immune system; however, dietary fatty acids in high-fat diets (HFDs) that induce obesity have consequences that are currently unclear regarding T-cell maintenance in bone marrow (BM). C57BL/6J mice were randomly assigned to isocaloric HFDs formulated with dietary fats rich in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), or MUFAs supplemented with eicosapentaenoic and docosahexaenoic acids for 20 weeks, followed by an analysis of the immunophenotypic feature of lymphocytes (CD3+) T and their subsets CD4+ and CD8+ T cells in spleen and BM, identification of fatty acids in BM extracellular fluid and analysis of the correspondence between fatty acids with the frequency of T-cell subsets in BM. Splenic CD3+ T cells were reduced irrespective of HFDs. In BM, CD3+ T cells were reduced after HFD-SFAs, while CD4+ T cells were increased after HFDs enriched in MUFAs and CD8+ T cells were reduced irrespective of HFDs. In BM extracellular fluid, the content of palmitic and myristic acids increased after HFD-SFAs and that of oleic acid increased after HFDs enriched in MUFAs. There was a statistical correspondence between HFD-induced changes in fatty acids in BM extracellular fluid and HFD-induced changes in the frequency of CD3+ and CD4+ T cells in BM. These findings reveal an undervalued critical role for dietary fatty acids in the selective acquisition of T-cell subsets in BM, highlighting that oleic acid existing in the surroundings of T-cell niches during HFD-induced obesity could be instrumental in the maintenance of CD4+ T cells.
YR 2022
FD 2022-05-21
LK http://hdl.handle.net/10668/22325
UL http://hdl.handle.net/10668/22325
LA en
NO Medrano M, Lemus-Conejo A, Lopez S, Millan-Linares MC, Rosillo MA, Muñiz M, et al. CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets. J Nutr Biochem. 2022 Sep;107:109057.
DS RISalud
RD Apr 6, 2025