RT Journal Article
T1 Differential Expression of MicroRNAs in Silent and Functioning Corticotroph Tumors.
A1 García-Martínez, Araceli
A1 Fuentes-Fayos, Antonio C
A1 Fajardo, Carmen
A1 Lamas, Cristina
A1 Cámara, Rosa
A1 López-Muñoz, Beatriz
A1 Aranda, Ignacio
A1 Luque, Raúl M
A1 Picó, Antonio
K1 corticotroph tumors
K1 miRNA
K1 pituitary neuroendocrine tumors
K1 transcriptional regulation
AB The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; of PKA, MAP3K8, MEK, MAPK3, NGFIB, NURR1, PITX1, and STAT3 were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively with TBX19 in silent CTs, a transcription factor related with the processing of POMC that can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.
SN 2077-0383
YR 2020
FD 2020-06-12
LK https://hdl.handle.net/10668/25813
UL https://hdl.handle.net/10668/25813
LA en
DS RISalud
RD Apr 8, 2025