RT Journal Article T1 Plasma lipid profiles discriminate bacterial from viral infection in febrile children. A1 Wang, Xinzhu A1 Nijman, Ruud A1 Camuzeaux, Stephane A1 Sands, Caroline A1 Jackson, Heather A1 Kaforou, Myrsini A1 Emonts, Marieke A1 Herberg, Jethro A A1 Maconochie, Ian A1 Carrol, Enitan D A1 Paulus, Stephane C A1 Zenz, Werner A1 Van der Flier, Michiel A1 de Groot, Ronald A1 Martinon-Torres, Federico A1 Schlapbach, Luregn J A1 Pollard, Andrew J A1 Fink, Colin A1 Kuijpers, Taco T A1 Anderson, Suzanne A1 Lewis, Matthew R A1 Levin, Michael A1 McClure, Myra A1 EUCLIDS consortium, AB Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The 'omics' approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics. YR 2019 FD 2019-11-27 LK http://hdl.handle.net/10668/14758 UL http://hdl.handle.net/10668/14758 LA en DS RISalud RD Apr 16, 2025