RT Journal Article T1 Bioactive Peptides from Lupin (Lupinus angustifolius) Prevent the Early Stages of Atherosclerosis in Western Diet-Fed ApoE-/- Mice. A1 Santos-Sanchez, Guillermo A1 Cruz-Chamorro, Ivan A1 Alvarez-Rios, Ana Isabel A1 Alvarez-Sanchez, Nuria A1 Rodriguez-Ortiz, Beatriz A1 Alvarez-Lopez, Ana Isabel A1 Fernandez-Pachon, Maria-Soledad A1 Pedroche, Justo A1 Millan, Francisco A1 Millan-Linares, Maria del Carmen A1 Lardone, Patricia Judith A1 Bejarano, Ignacio A1 Carrillo-Vico, Antonio K1 Alcalase K1 atherosclerosis K1 bioactive peptides K1 cholesterol K1 inflammation K1 lupin K1 oxidative stress AB We have previously reported the in vitro hypocholesterolemic, anti-inflammatory, and antioxidant effects of Alcalase-generated lupin protein hydrolysate (LPH). Given that lipoprotein deposition, oxidative stress, and inflammation are the main components of atherogenesis, we characterized the LPH composition, in silico identified LPH-peptides with activities related to atherosclerosis, and evaluated the in vivo LPH effects on atherosclerosis risk factors in a mouse model of atherosclerosis. After 15 min of Alcalase hydrolysis, peptides smaller than 8 kDa were obtained, and 259 peptides out of 278 peptides found showed biological activities related to atherosclerosis risk factors. Furthermore, LPH administration for 12 weeks reduced the plasma lipids, as well as the cardiovascular and atherogenic risk indexes. LPH also increased the total antioxidant capacity, decreased endothelial permeability, inflammatory response, and atherogenic markers. Therefore, this study describes for the first time that LPH prevents the early stages of atherosclerosis. PB American Chemical Society YR 2022 FD 2022-06-29 LK http://hdl.handle.net/10668/22587 UL http://hdl.handle.net/10668/22587 LA en NO Santos-Sánchez G, Cruz-Chamorro I, Álvarez-Ríos AI, Álvarez-Sánchez N, Rodríguez-Ortiz B, Álvarez-López AI, et al. Bioactive Peptides from Lupin (Lupinus angustifolius) Prevent the Early Stages of Atherosclerosis in Western Diet-Fed ApoE-/- Mice. J Agric Food Chem. 2022 Jul 13;70(27):8243-8253. DS RISalud RD Apr 6, 2025