RT Journal Article
T1 Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development.
A1 Mora-Lorca, José Antonio
A1 Sáenz-Narciso, Beatriz
A1 Gaffney, Christopher J
A1 Naranjo-Galindo, Francisco José
A1 Pedrajas, José Rafael
A1 Guerrero-Gómez, David
A1 Dobrzynska, Agnieszka
A1 Askjaer, Peter
A1 Szewczyk, Nathaniel J
A1 Cabello, Juan
A1 Miranda-Vizuete, Antonio
K1 Caenorhabditis elegans
K1 Embryonic development
K1 Glutathione reductase
K1 Mitochondria
K1 Redox
AB Glutathione is the most abundant thiol in the vast majority of organisms and is maintained in its reduced form by the flavoenzyme glutathione reductase. In this work, we describe the genetic and functional analysis of the Caenorhabditis elegans gsr-1 gene that encodes the only glutathione reductase protein in this model organism. By using green fluorescent protein reporters we demonstrate that gsr-1 produces two GSR-1 isoforms, one located in the cytoplasm and one in the mitochondria. gsr-1 loss of function mutants display a fully penetrant embryonic lethal phenotype characterized by a progressive and robust cell division delay accompanied by an aberrant distribution of interphasic chromatin in the periphery of the cell nucleus. Maternally expressed GSR-1 is sufficient to support embryonic development but these animals are short-lived, sensitized to chemical stress, have increased mitochondrial fragmentation and lower mitochondrial DNA content. Furthermore, the embryonic lethality of gsr-1 worms is prevented by restoring GSR-1 activity in the cytoplasm but not in mitochondria. Given the fact that the thioredoxin redox systems are dispensable in C. elegans, our data support a prominent role of the glutathione reductase/glutathione pathway in maintaining redox homeostasis in the nematode.
YR 2016
FD 2016-04-24
LK http://hdl.handle.net/10668/10024
UL http://hdl.handle.net/10668/10024
LA en
DS RISalud
RD Apr 7, 2025