RT Journal Article
T1 The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection
A1 Sabido-Bozo, Susana
A1 Perez-Linero, Ana Maria
A1 Manzano-Lopez, Javier
A1 Rodriguez-Gallardo, Sofia
A1 Aguilera-Romero, Auxiliadora
A1 Cortes-Gomez, Alejandro
A1 Lopez, Sergio
A1 Wellinger, Ralf Erik
A1 Muñiz, Manuel
K1 P24 complex
K1 COPI
K1 Golgi
K1 ArfGEFs
K1 Arf1
K1 Proteína coat de complejo I
K1 Aparato de Golgi
K1 Factores de intercambio de guanina nucleótido
K1 Factor 1 de ribosilacion-ADP
AB Golgi trafficking depends on the small GTPase Arf1 which, upon activation, drives the assembly of different coats onto budding vesicles. Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites. In yeast, Gea1 in the cis-Golgi and Gea2 in the medial-Golgi activate Arf1 to form COPI-coated vesicles for retrograde cargo sorting, whereas Sec7 generates clathrin/adaptor-coated vesicles at the trans-Golgi network (TGN) for forward cargo transport. A central question is how the same activated Arf1 protein manages to assemble different coats depending on the donor Golgi compartment. A previous study has postulated that the interaction between Gea1 and COPI would channel Arf1 activation for COPI vesicle budding. Here, we found that the p24 complex, a major COPI vesicle cargo, promotes the binding of Gea1 with COPI by increasing the COPI association to the membrane independently of Arf1 activation. Furthermore, the p24 complex also facilitates the interaction of Arf1 with its COPI effector. Therefore, our study supports a mechanism by which the p24 complex contributes to program Arf1 activation by Gea1 for selective COPI coat assembly at the cis-Golgi compartment.
PB MDPI
SN 1661-6596
YR 2021
FD 2021-01-03
LK http://hdl.handle.net/10668/4180
UL http://hdl.handle.net/10668/4180
LA en
NO Sabido-Bozo S, Perez-Linero AM, Manzano-Lopez J, Rodriguez-Gallardo S, Aguilera-Romero A, Cortes-Gomez A, et al. The p24 Complex Contributes to Specify Arf1 for COPI Coat Selection. Int J Mol Sci. 2021 Jan 3;22(1):423
DS RISalud
RD Apr 17, 2025