RT Journal Article
T1 The Impact of Melatonin and NLRP3 Inflammasome on the Expression of microRNAs in Aged Muscle.
A1 Sayed, Ramy Ka
A1 Fernández-Ortiz, Marisol
A1 Fernández-Martínez, José
A1 Aranda Martínez, Paula
A1 Guerra-Librero, Ana
A1 Rodríguez-Santana, César
A1 de Haro, Tomás
A1 Escames, Germaine
A1 Acuña-Castroviejo, Darío
A1 Rusanova, Iryna
K1 NF-kB
K1 NLRP3 inflammasome
K1 aging
K1 melatonin
K1 microRNAs
AB Muscular aging is a complex process and underlying physiological mechanisms are not fully clear. In recent years, the participation of the NF-kB pathway and the NLRP3 inflammasome in the chronic inflammation process that accompanies the skeletal muscle's aging has been confirmed. microRNAs (miRs) form part of a gene regulatory machinery, and they control numerous biological processes including inflammatory pathways. In this work, we studied the expression of four miRs; three of them are considered as inflammatory-related miRs (miR-21, miR-146a, and miR-223), and miR-483, which is related to the regulation of melatonin synthesis, among other targets. To investigate the changes of miRs expression in muscle along aging, the impact of inflammation, and the role of melatonin in aged skeletal muscle, we used the gastrocnemius muscle of wild type (WT) and NLRP3-knockout (NLRP3-) mice of 3, 12, and 24 months-old, with and without melatonin supplementation. The expression of miRs and pro-caspase-1, caspase-3, pro-IL-1β, bax, bcl-2, and p53, was investigated by qRT-PCR analysis. Histological examination of the gastrocnemius muscle was also done. The results showed that age increased the expression of miR-21 (p
SN 2076-3921
YR 2021
FD 2021-03-27
LK https://hdl.handle.net/10668/26093
UL https://hdl.handle.net/10668/26093
LA en
DS RISalud
RD Apr 12, 2025