RT Journal Article
T1 Mesenchymal Stem Cells From Adipose Tissue Do not Improve Functional Recovery After Ischemic Stroke in Hypertensive Rats.
A1 Diekhorst, Luke
A1 Gomez-de Frutos, Mari Carmen
A1 Laso-Garcia, Fernando
A1 Otero-Ortega, Laura
A1 Fuentes, Blanca
A1 Jolkkonen, Jukka
A1 Detante, Olivier
A1 Moisan, Anaick
A1 Leyva, Laura
A1 Martinez-Arroyo, Arturo
A1 Diez-Tejedor, Exuperio
A1 Gutierrez-Fernandez, Maria
K1 Brain
K1 Comorbidity
K1 Hypertension
K1 Neurogenesis
K1 Stem cells
AB Background and Purpose- Hypertension is the most frequent comorbidity in stroke.The purpose of this study was to evaluate whether hypertension alters the response to treatment with adipose tissue-derived mesenchymal stem cells (ADMSCs) after an ischemic stroke in rats. Methods- Ischemic stroke was induced in male normotensive or hypertensive rats. Either vehicle or 1×106 ADMSC was intravenously administered at 48 hours poststroke. Functional outcome, lesion size and volume, and markers of brain repair (GFAP [glial fibrillary acidic protein], doublecortin, CD-31, α-smooth muscle actin) were evaluated. Results- Hypertensive rats had larger lesions, higher apparent diffusion coefficients (ADC) and worse functional outcomes than normotensive rats. Hypertension increased GFAP and vascular markers (CD-31 and α-smooth muscle actin). The hypertensive rats treated with ADMSC did not show any significant improvement in functional recovery, lesion size, ADC values, or histological markers compared with those which received the vehicle. Conclusions- ADMSC did not reverse the hypertension-induced increase in lesion severity or functional impairment. Gliosis, neurogenesis, or vascular markers were not affected by ADMSC in hypertensive rats. Hypertension has a negative impact on the therapeutic effect of ADMSC after an ischemic stroke.
PB Lippincott Williams & Wilkins
YR 2019
FD 2019-10-10
LK http://hdl.handle.net/10668/14647
UL http://hdl.handle.net/10668/14647
LA en
NO Diekhorst L, Gómez-de Frutos MC, Laso-García F, Otero-Ortega L, Fuentes B, Jolkkonen J, et al. Mesenchymal Stem Cells From Adipose Tissue Do not Improve Functional Recovery After Ischemic Stroke in Hypertensive Rats. Stroke. 2020 Jan;51(1):342-346
NO This work was sponsored by the REgenerative Stem cell therapy forSTroke in Europe (RESSTORE) project (www.resstore.eu) fundedby the European Commission under the Horizon2020 program (grantnumber 681044), Miguel Servet (CP15/00069, María Gutiérrez-Fernández), predoctoral fellowship (FI17/00188, MariCarmenGómez-de Frutos), Spanish Research Network on CerebrovascularDiseases (INVICTUS PLUS-RD16/0019/0005) of the Carlos III HealthInstitute, Ministry of Science and Innovation of Spain and the EuropeanRegional Development Fund (FEDER Funding) and Juan de la Ciervafellowship (IJCI-2017-33505,Laura Otero-Ortega), (http://ec.europa.eu/esf). Blanca Fuentes: Financial activities outside the submitted work:Novo Nordisk, Bayer, BMS-Pzifer, Novartis, and Daiichi Sankyo.
DS RISalud
RD Apr 28, 2025