RT Journal Article
T1 Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
A1 Visiedo, Francisco
A1 Santos-Rosendo, Celeste
A1 Mateos-Bernal, Rosa M.
A1 del Mar Gil-Sanchez, M.
A1 Bugatto, Fernando
A1 Aguilar-Diosdado, Manuel
A1 Segundo, Carmen
A1 Lopez-Tinoco, Cristina
K1 Protein s-nitrosylation
K1 Nitric-oxide
K1 Oxidative stress
K1 Apoptosis
K1 Pathophysiology
K1 Trophoblasts
K1 Expression
K1 Erk1/2
K1 P38
AB Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n = 8) and GDM (n = 8) pregnancies. S-Nitrosylation was measured using the biotin-switch assay, while the expression and protein activity were assessed by immunoblotting and colorimetric methods, respectively. Results indicated that catalase and peroxiredoxin nitrosylation levels were greater in GDM placentas, and that was accompanied by reduced catalase activity. S-Nitrosylation of ERK1/2 and AKT was increased in GDM placentas, and their activities were inhibited. Activities of caspase-3 and caspase-9 were increased, with the latter also showing diminished nitrosylation levels. These findings suggest that S-nitrosylation is a little-known, but critical, mechanism by which NO directlymodulates key placental proteins in women with GDM and, as a consequence, maternal and fetal anomalies during pregnancy can occur.
PB Hindawi ltd
SN 1942-0900
YR 2017
FD 2017-01-01
LK http://hdl.handle.net/10668/19062
UL http://hdl.handle.net/10668/19062
LA en
DS RISalud
RD Apr 19, 2025