RT Journal Article
T1 Amphidinol 22, a New Cytotoxic and Antifungal Amphidinol from the Dinoflagellate Amphidinium carterae.
A1 Martínez, Kevin A
A1 Lauritano, Chiara
A1 Druka, Dana
A1 Romano, Giovanna
A1 Grohmann, Teresa
A1 Jaspars, Marcel
A1 Martín, Jesús
A1 Díaz, Caridad
A1 Cautain, Bastien
A1 de la Cruz, Mercedes
A1 Ianora, Adrianna
A1 Reyes, Fernando
K1 amphidinol
K1 anticancer
K1 antifungal
K1 bioactive compounds
K1 blue biotechnology
K1 dinoflagellates
K1 marine microalgae
K1 marine natural products (MNPs)
AB Due to the unique biodiversity and the physical-chemical properties of their environment, marine microorganisms have evolved defense and signaling compounds that often have no equivalent in terrestrial habitats. The aim of this study was to screen extracts of the dinoflagellate Amphidinium carterae for possible bioactivities (i.e., anticancer, anti-inflammatory, anti-diabetes, antibacterial and antifungal properties) and identify bioactive compounds. Anticancer activity was evaluated on human lung adenocarcinoma (A549), human skin melanoma (A2058), human hepatocellular carcinoma (HepG2), human breast adenocarcinoma (MCF7) and human pancreas carcinoma (MiaPaca-2) cell lines. Antimicrobial activities were evaluated against Gram-positive bacteria (Staphylococcus aureus MRSA and MSSA), Gram-negative bacteria (i.e., Escherichia coli and Klebsiella pneumoniae), Mycobacterium tuberculosis and the fungus Aspergillus fumigatus. The results indicated moderate biological activities against all the cancer cells lines and microorganisms tested. Bioassay-guided fractionation assisted by HRMS analysis allowed the detection of one new and two known amphidinols that are potentially responsible for the antifungal and cytotoxic activities observed. Further isolation, purification and structural elucidation led to a new amphidinol, named amphidinol 22. The planar structure of the new compound was determined by analysis of its HRMS and 1D and 2D NMR spectra. Its biological activity was evaluated, and it displayed both anticancer and antifungal activities.
YR 2019
FD 2019-06-27
LK http://hdl.handle.net/10668/14195
UL http://hdl.handle.net/10668/14195
LA en
DS RISalud
RD Apr 17, 2025