RT Journal Article
T1 miRNAs as radio-response biomarkers for breast cancer stem cells
A1 Griñán-Lisón, Carmen
A1 Olivares-Urbano, María Auxiliadora
A1 Jiménez, Gema
A1 López-Ruiz, Elena
A1 Del Val, Coral
A1 Morata-Tarifa, Cynthia
A1 Entrena, José Manuel
A1 González-Ramírez, Amanda Rocío
A1 Boulaiz, Houria
A1 Zurita Herrera, Mercedes
A1 Núñez, María Isabel
A1 Marchal, Juan Antonio
K1 Biomarkers
K1 Breast cancer
K1 CSCs
K1 miRNAs
K1 Radiation
K1 Radiotherapy
K1 Biomarcadores
K1 Neoplasias de la mama
K1 MicroARNs
K1 Radiación
K1 Radioterapia
AB In breast cancer (BC), the presence of cancer stem cells (CSCs) has been related to relapse, metastasis, and radioresistance. Radiotherapy (RT) is an extended BC treatment, but is not always effective. CSCs have several mechanisms of radioresistance in place, and some miRNAs are involved in the cellular response to ionizing radiation (IR). Here, we studied how IR affects the expression of miRNAs related to stemness in different molecular BC subtypes. Exposition of BC cells to radiation doses of 2, 4, or 6 Gy affected their phenotype, functional characteristics, pluripotency gene expression, and in vivo tumorigenic capacity. This held true for various molecular subtypes of BC cells (classified by ER, PR and HER-2 status), and for BC cells either plated in monolayer, or being in suspension as mammospheres. However, the effect of IR on the expression of eight stemness- and radioresistance-related miRNAs (miR-210, miR-10b, miR-182, miR-142, miR-221, miR-21, miR-93, miR-15b) varied, depending on cell line subpopulation and clinicopathological features of BC patients. Therefore, clinicopathological features and, potentially also, chemotherapy regimen should be both taken into consideration, for determining a potential miRNA signature by liquid biopsy in BC patients treated with RT. Personalized and precision RT dosage regimes could improve the prognosis, treatment, and survival of BC patients.
PB FEBS Press and John Wiley & Sons
SN 1574-7891
YR 2020
FD 2020-02-06
LK http://hdl.handle.net/10668/3932
UL http://hdl.handle.net/10668/3932
LA en
NO Griñán-Lisón C, Olivares-Urbano MA, Jiménez G, López-Ruiz E, Del Val C, Morata-Tarifa C, et al. miRNAs as radio-response biomarkers for breast cancer stem cells. Mol Oncol. 2020 Mar;14(3):556-570
DS RISalud
RD Apr 6, 2025