RT Journal Article
T1 Azole resistance survey on clinical Aspergillus fumigatus isolates in Spain.
A1 Escribano, Pilar
A1 Rodriguez-Sanchez, Belen
A1 Diaz-Garcia, Judith
A1 Martin-Gomez, Maria Teresa
A1 Ibañez-Martinez, Elisa
A1 Rodriguez-Mayo, Maria
A1 Pelaez, Teresa
A1 Garcia-Gomez de la Pedrosa, Elia
A1 Tejero-Garcia, Rocio
A1 Marimon, Jose Maria
A1 Reigadas, Elena
A1 Rezusta, Antonio
A1 Labayru-Echeverría, Cristina
A1 Perez-Ayala, Ana
A1 Ayats, Josefina
A1 Cobo, Fernando
A1 Pazos, Carmen
A1 Lopez-Soria, Leyre
A1 Alastruey-Izquierdo, Ana
A1 Muñoz, Patricia
A1 Guinea, Jesus
K1 Aspergillus fumigatus
K1 Azole
K1 Isavuconazole
K1 Resistance
K1 Voriconazole
AB We aimed to assess the percentage of azole resistance in Aspergillus fumigatus in Spain. Thirty participating Spanish hospitals stored all morphologically identified A. fumigatus sensu lato clinical isolates-regardless their clinical significance-from 15 February to 14 May 2019. Isolates showing azole resistance according to the EUCAST 9.3.2 methodology were molecularly identified and the cyp51A gene was studied in A. fumigatus sensu stricto isolates. Eight hundred and forty-seven isolates from 725 patients were collected in 29 hospitals (A. fumigatus sensu stricto (n = 828) and cryptic species (n = 19)). Isolates were mostly from the lower respiratory tract (94.0%; 797/847). Only cryptic species were amphotericin B resistant. Sixty-three (7.4%) out of the 847 isolates were resistant to ≥1 azole(s). Azole resistance was higher in cryptic species than in A. fumigatus sensu stricto (95%, 18/19 vs. 5.5%, 45/828); isavuconazole was associated to the lowest number of non-wild type isolates. The dominant mechanism of resistance was the presence of TR34-L98H substitutions (n = 24 out of 63). Out of the 725 patients, 48 (6.6%) carried either cryptic species (n = 14) or A. fumigatus sensu stricto (n = 34; 4.7%) resistant isolates. Aspergillus fumigatus sensu stricto harbouring either the TR34-L98H (n = 19) or TR46/Y121F/T289A (n = 1) mutations were detected in patients in hospitals located at 7/24 studied cities. Of the patients, 6.6% carry azole-resistant A. fumigatus sensu lato isolates in Spain. TR34-L98H is the dominant cyp51A gene substitutions, although its presence is not widespread.
PB Elsevier
YR 2020
FD 2020-09-22
LK http://hdl.handle.net/10668/16362
UL http://hdl.handle.net/10668/16362
LA en
NO Escribano P, Rodríguez-Sánchez B, Díaz-García J, Martín-Gómez MT, Ibáñez-Martínez E, Rodríguez-Mayo M, et al. Azole resistance survey on clinical Aspergillus fumigatus isolates in Spain. Clin Microbiol Infect. 2021 Aug;27(8):1170.e1-1170.e7
DS RISalud
RD Apr 11, 2025