%0 Journal Article
%A Bellocchi, Chiara
%A Fernández-Ochoa, Álvaro
%A Montanelli, Gaia
%A Vigone, Barbara
%A Santaniello, Alessandro
%A Quirantes-Piné, Rosa
%A Borrás-Linares, Isabel
%A Gerosa, Maria
%A Artusi, Carolina
%A Gualtierotti, Roberta
%A Segura-Carrettero, Antonio
%A Alarcón-Riquelme, Marta E
%A Beretta, Lorenzo
%T Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases.
%D 2019
%@ 2077-0383
%U http://hdl.handle.net/10668/14445
%X Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren's syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs.
%K Sjögren’s syndrome
%K metabolomics
%K microbiomic
%K primary anti-phosholipid syndrome
%K systemic autoimmune diseases
%K systemic lupus erythematosus
%K undifferentiated connective tissue diseases
%~