RT Journal Article
T1 Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.
A1 Messina, Luciano
A1 Gavira, Jose A
A1 Pernagallo, Salvatore
A1 Unciti-Broceta, Juan D
A1 Sanchez Martin, Rosario M
A1 Diaz-Mochon, Juan J
A1 Vaccaro, Susanna
A1 Conejero-Muriel, Mayte
A1 Pineda-Molina, Estela
A1 Caruso, Salvatore
A1 Musumeci, Luca
A1 Di Pasquale, Roberta
A1 Pontillo, Angela
A1 Sincinelli, Francesca
A1 Pavan, Mauro
A1 Secchieri, Cynthia
K1 Streptomyces koganeiensis
K1 hyaluronic acid or hyaluronan
K1 hyaluronidase
AB Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial-scale recombinant production, purification and full characterization, including X-ray structure determination at 1.45 Å, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis. The recombinant S. koganeiensis Hyal (rHyal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rHyal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross-infection.
YR 2016
FD 2016-07-04
LK http://hdl.handle.net/10668/10184
UL http://hdl.handle.net/10668/10184
LA en
DS RISalud
RD Apr 11, 2025