%0 Journal Article
%A Messina, Luciano
%A Gavira, Jose A
%A Pernagallo, Salvatore
%A Unciti-Broceta, Juan D
%A Sanchez Martin, Rosario M
%A Diaz-Mochon, Juan J
%A Vaccaro, Susanna
%A Conejero-Muriel, Mayte
%A Pineda-Molina, Estela
%A Caruso, Salvatore
%A Musumeci, Luca
%A Di Pasquale, Roberta
%A Pontillo, Angela
%A Sincinelli, Francesca
%A Pavan, Mauro
%A Secchieri, Cynthia
%T Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.
%D 2016
%U http://hdl.handle.net/10668/10184
%X Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial-scale recombinant production, purification and full characterization, including X-ray structure determination at 1.45 Å, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis. The recombinant S. koganeiensis Hyal (rHyal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rHyal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross-infection.
%K Streptomyces koganeiensis
%K hyaluronic acid or hyaluronan
%K hyaluronidase
%~