RT Journal Article
T1 Understanding the Adult Mammalian Heart at Single-Cell RNA-Seq Resolution.
A1 Marín-Sedeño, Ernesto
A1 de Morentin, Xabier Martínez
A1 Pérez-Pomares, Jose M
A1 Gómez-Cabrero, David
A1 Ruiz-Villalba, Adrián
K1 cardiac cell heterogeneity
K1 heart
K1 infarction
K1 single-cell RNAseq
K1 transcriptomics
AB During the last decade, extensive efforts have been made to comprehend cardiac cell genetic and functional diversity. Such knowledge allows for the definition of the cardiac cellular interactome as a reasonable strategy to increase our understanding of the normal and pathologic heart. Previous experimental approaches including cell lineage tracing, flow cytometry, and bulk RNA-Seq have often tackled the analysis of cardiac cell diversity as based on the assumption that cell types can be identified by the expression of a single gene. More recently, however, the emergence of single-cell RNA-Seq technology has led us to explore the diversity of individual cells, enabling the cardiovascular research community to redefine cardiac cell subpopulations and identify relevant ones, and even novel cell types, through their cell-specific transcriptomic signatures in an unbiased manner. These findings are changing our understanding of cell composition and in consequence the identification of potential therapeutic targets for different cardiac diseases. In this review, we provide an overview of the continuously changing cardiac cellular landscape, traveling from the pre-single-cell RNA-Seq times to the single cell-RNA-Seq revolution, and discuss the utilities and limitations of this technology.
SN 2296-634X
YR 2021
FD 2021-05-12
LK https://hdl.handle.net/10668/24714
UL https://hdl.handle.net/10668/24714
LA en
DS RISalud
RD Apr 17, 2025