RT Journal Article
T1 iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation
A1 Lopez-Caraballo, Lidia
A1 Martorell-Marugan, Jordi
A1 Carmona-Sáez, Pedro
A1 Gonzalez-Munoz, Elena
K1 Secondary progressive multiple sclerosis (SPMS)
K1 Disease modeling
K1 iPSCs
K1 Oligodendrocyte progenitor cells (OPCs)
K1 Secretome
K1 Cell migration
K1 Esclerosis múltiple crónica progresiva
K1 Células precursoras de oligodendrocitos
K1 Movimiento celular
K1 Remielinización
AB The most challenging aspect of secondary progressive multiple sclerosis (SPMS) is the lack of efficient regenerative response for remyelination, which is carried out by the endogenous population of adult oligoprogenitor cells (OPCs) after proper activation. OPCs must proliferate and migrate to the lesion and then differentiate into mature oligodendrocytes. To investigate the OPC cellular component in SPMS, we developed induced pluripotent stem cells (iPSCs) from SPMS-affected donors and age-matched controls (CT). We confirmed their efficient and similar OPC differentiation capacity, although we reported SPMS-OPCs were transcriptionally distinguishable from their CT counterparts. Analysis of OPC-generated conditioned media (CM) also evinced differences in protein secretion. We further confirmed SPMS-OPC CM presented a deficient capacity to stimulate OPC in vitro migration that can be compensated by exogenous addition of specific components. Our results provide an SPMS-OPC cellular model and encouraging venues to study potential cell communication deficiencies in the progressive form of multiple sclerosis (MS) for future treatment strategies.
PB MDPI
YR 2020
FD 2020-07-29
LK http://hdl.handle.net/10668/3558
UL http://hdl.handle.net/10668/3558
LA en
NO Lopez-Caraballo L, Martorell-Marugan J, Carmona-Sáez P, Gonzalez-Munoz E. iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation. Cells. 2020 Jul 29;9(8):1803
DS RISalud
RD Apr 15, 2025