RT Journal Article
T1 Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry
A1 Contreras, Maria del Mar
A1 Bribi, Noureddine
A1 Maria Gomez-Caravaca, Ana
A1 Galvez, Julio
A1 Segura-Carretero, Antonio
K1 Isoquinoline alkaloids
K1 Electrospray-ionization
K1 Identification
K1 Metabolism
K1 Protopine
K1 Extracts
K1 Fruits
K1 Ms/ms
K1 Model
AB Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matching their accurate mass signals and suggested molecular formulae with those previously reported in libraries and databases. Moreover, the proposed structures were corroborated by studying their fragmentation pattern obtained by both platforms. In this way, 8 and 26 isoquinoline alkaloids were characterized using GC-QTOF-MS and RP-UHPLC-DAD-QTOFMS, respectively, and they belonged to the following subclasses: protoberberine, protopine, aporphine, benzophenanthridine, spirobenzylisoquinoline, morphinandienone, and benzylisoquinoline. Moreover, the latter analytical method was selected to determine at 280nm the concentration of protopine (9.6 +/- 0.7mg/g), a potential active compound of the extract. In conclusion, although GC- MS has been commonly used for the analysis of this type of phytochemicals, RP-UHPLC-DAD-QTOF-MS provided essential complementary information. This analytical method can be applied for the quality control of phytopharmaceuticals containing Fumaria extracts currently found in the market.
PB Hindawi ltd
SN 1687-8760
YR 2017
FD 2017-07-02
LK http://hdl.handle.net/10668/19060
UL http://hdl.handle.net/10668/19060
LA en
NO Contreras MDM, Bribi N, Gómez-Caravaca AM, Gálvez J, Segura-Carretero A. Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry. Int J Anal Chem. 2017;2017:5178729.
DS RISalud
RD Apr 18, 2025