RT Journal Article
T1 Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice.
A1 Rodriguez, Maria J
A1 Wangorsch, Andrea
A1 Gomez, Francisca
A1 Schulke, Stefan
A1 Torres, Maria J
A1 Vieths, Stefan
A1 Scheurer, Stephan
A1 Toda, Masako
A1 Mayorga, Cristobalina
K1 Prunus persica
K1 T-Lymphocytes, Helper-Inducer
K1 T-Lymphocytes, Regulatory
K1 Th2 Cells
AB The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.
PB John Wiley & Sons
YR 2018
FD 2018-06-13
LK http://hdl.handle.net/10668/12711
UL http://hdl.handle.net/10668/12711
LA en
NO Rodriguez MJ, Wangorsch A, Gomez F, Schülke S, Torres MJ, Vieths S, et al. Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice. J Immunol Res. 2018 Jun 13;2018:3479185
NO The study was funded by the Institute of Health “Carlos III” of the Ministry of Economy and Competitiveness (PI15/ 00559), RETICS ARADyAL (RD1612/0006/0001), Program Juan Rodés, 2016 (JR/00034), Andalusian Regional Ministry of Economy and Knowledge (CTS-7433), and Nicolas Monardes Program (C-0044-2012 SAS2013). The grants were cofunded by the European Regional Development Fund (ERDF).
DS RISalud
RD Apr 18, 2025