RT Journal Article
T1 Methylation alterations are not a major cause of PTTG1 misregulation.
A1 Hidalgo, Manuel
A1 Galan, Jose Jorge
A1 Sáez, Carmen
A1 Ferrero, Eduardo
A1 Castilla, Carolina
A1 Ramirez-Lorca, Reposo
A1 Pelaez, Pablo
A1 Ruiz, Agustin
A1 Japón, Miguel A
A1 Royo, Jose Luis
K1 Técnicas de Cultivo de Célula
K1 Regulación Neoplásica de la Expresión Génica
K1 Proteínas de Neoplasias
K1 Línea Celular Tumoral
K1 Metilación de ADN
K1 Epigénesis Genética
AB BACKGROUNDOn its physiological cellular context, PTTG1 controls sister chromatid segregation during mitosis. Within its crosstalk to the cellular arrest machinery, relies a checkpoint of integrity for which gained the over name of securin. PTTG1 was found to promote malignant transformation in 3T3 fibroblasts, and further found to be overexpressed in different tumor types. More recently, PTTG1 has been also related to different processes such as DNA repair and found to trans-activate different cellular pathways involving c-myc, bax or p53, among others. PTTG1 over-expression has been correlated to a worse prognosis in thyroid, lung, colorectal cancer patients, and it can not be excluded that this effect may also occur in other tumor types. Despite the clinical relevance and the increasing molecular characterization of PTTG1, the reason for its up-regulation remains unclear.METHODWe analysed PTTG1 differential expression in PC-3, DU-145 and LNCaP tumor cell lines, cultured in the presence of the methyl-transferase inhibitor 5-Aza-2'-deoxycytidine. We also tested whether the CpG island mapping PTTG1 proximal promoter evidenced a differential methylation pattern in differentiated thyroid cancer biopsies concordant to their PTTG1 immunohistochemistry status. Finally, we performed whole-genome LOH studies using Affymetix 50 K microarray technology and FRET analysis to search for allelic imbalances comprising the PTTG1 locus.CONCLUSIONOur data suggest that neither methylation alterations nor LOH are involved in PTTG1 over-expression. These data, together with those previously reported, point towards a post-transcriptional level of misregulation associated to PTTG1 over-expression.
PB BioMed Central Ltd
YR 2008
FD 2008-04-21
LK http://hdl.handle.net/10668/1762
UL http://hdl.handle.net/10668/1762
LA en
NO Hidalgo M, Galan JJ, Sáez C, Ferrero E, Castilla C, Ramirez-Lorca R, et al. Methylation alterations are not a major cause of PTTG1 misregulation. BMC Cancer. 2008; 8:110
NO Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 11, 2025