RT Journal Article
T1 Soluble Receptor Isoform of IFN-Beta (sIFNAR2) in Multiple Sclerosis Patients and Their Association With the Clinical Response to IFN-Beta Treatment
A1 Aliaga-Gaspar, Pablo
A1 Hurtado-Guerrero, Isaac
A1 Ciano-Petersen, Nicolas Lundahl
A1 Urbaneja, Patricia
A1 Brichette-Mieg, Isabel
A1 Reyes, Virginia
A1 Rodriguez-Bada, Jose Luis
A1 Alvarez-Lafuente, Roberto
A1 Arroyo, Rafael
A1 Quintana, Ester
A1 Ramio-Torrenta, Lluis
A1 Alonso, Ana
A1 Leyva, Laura
A1 Fernandez, Oscar
A1 Oliver-Martos, Begona
K1 alternative splicing
K1 soluble receptors
K1 IFNAR
K1 interferon beta
K1 multiple sclerosis
K1 I interferon receptor
K1 Ifnar-2
K1 Serum
K1 Validation
K1 Cytokines
K1 Subunit
AB PurposeInterferon beta receptor 2 subunit (IFNAR2) can be produced as a transmembrane protein, but also as a soluble form (sIFNAR2) generated by alternative splicing or proteolytic cleavage, which has both agonist and antagonist activities for IFN-beta. However, its role regarding the clinical response to IFN-beta for relapsing-remitting multiple sclerosis (RRMS) is unknown. We aim to evaluate the in vitro short-term effects and after 6 and 12 months of IFN-beta therapy on sIFNAR2 production and their association with the clinical response in MS patients. MethodsNinety-four RRMS patients were included and evaluated at baseline, 6 and 12 months from treatment onset. A subset of 41 patients were classified as responders and non-responders to IFN-beta therapy. sIFNAR2 serum levels were measured by ELISA. mRNA expression for IFNAR1, IFNAR2 splice variants, MxA and proteases were assessed by RT-PCR. The short-term effect was evaluated in PBMC from RRMS patients after IFN-beta stimulation in vitro. ResultsProtein and mRNA levels of sIFNAR2 increased after IFN-beta treatment. According to the clinical response, only non-responders increased sIFNAR2 significantly at both protein and mRNA levels. sIFNAR2 gene expression correlated with the transmembrane isoform expression and was 2.3-fold higher. While MxA gene expression increased significantly after treatment, IFNAR1 and IFNAR2 only slightly increased. After short-term IFN-beta in vitro induction of PBMC, 6/7 patients increased the sIFNAR2 expression. ConclusionsIFN-beta administration induces the production of sIFNAR2 in RRMS and higher levels might be associated to the reduction of therapeutic response. Thus, levels of sIFNAR2 could be monitored to optimize an effective response to IFN-beta therapy.
PB Frontiers media sa
SN 1664-3224
YR 2021
FD 2021-12-16
LK https://hdl.handle.net/10668/24480
UL https://hdl.handle.net/10668/24480
LA en
DS RISalud
RD Apr 5, 2025