RT Journal Article
T1 LINE-1 transcription in round spermatids is associated with accretion of 5-carboxylcytosine in their open reading frames.
A1 Blythe, Martin J
A1 Kocer, Ayhan
A1 Rubio-Roldan, Alejandro
A1 Giles, Tom
A1 Abakir, Abdulkadir
A1 Ialy-Radio, Côme
A1 Wheldon, Lee M
A1 Bereshchenko, Oxana
A1 Bruscoli, Stefano
A1 Kondrashov, Alexander
A1 Drevet, Joël R
A1 Emes, Richard D
A1 Johnson, Andrew D
A1 McCarrey, John R
A1 Gackowski, Daniel
A1 Olinski, Ryszard
A1 Cocquet, Julie
A1 Garcia-Perez, Jose L
A1 Ruzov, Alexey
AB Chromatin of male and female gametes undergoes a number of reprogramming events during the transition from germ cell to embryonic developmental programs. Although the rearrangement of DNA methylation patterns occurring in the zygote has been extensively characterized, little is known about the dynamics of DNA modifications during spermatid maturation. Here, we demonstrate that the dynamics of 5-carboxylcytosine (5caC) correlate with active transcription of LINE-1 retroelements during murine spermiogenesis. We show that the open reading frames of active and evolutionary young LINE-1s are 5caC-enriched in round spermatids and 5caC is eliminated from LINE-1s and spermiogenesis-specific genes during spermatid maturation, being simultaneously retained at promoters and introns of developmental genes. Our results reveal an association of 5caC with activity of LINE-1 retrotransposons suggesting a potential direct role for this DNA modification in fine regulation of their transcription.
YR 2021
FD 2021-06-07
LK http://hdl.handle.net/10668/17974
UL http://hdl.handle.net/10668/17974
LA en
DS RISalud
RD Apr 12, 2025