RT Journal Article
T1 Natural Extracts Abolished Lipid Accumulation in Cells Harbouring non-favourable PNPLA3 genotype
A1 Rojas, Angela
A1 Gallego, Paloma
A1 Gil-Gomez, Antonio
A1 Munoz-Hernandez, Rocio
A1 Rojas, Lourdes
A1 Maldonado, Rosario
A1 Gallego-Duran, Rocio
A1 Garcia-Valdecasas, Marta
A1 Del Campo, Jose A.
A1 Bautista, Juan D.
A1 Romero-Gomez, Manuel
K1 Steatosis
K1 Lipid droplets
K1 Triglycerides
K1 Quercetin
K1 Cynara scolymus
K1 Agaricus Bisporus
K1 Mushroom
K1 Artichoke
K1 Fatty liver-disease
K1 Hepatic steatosis
K1 Insulin-resistance
K1 Hepg2 cells
K1 In-vitro
K1 Acid
K1 Resveratrol
K1 Lipogenesis
K1 Activation
K1 Expression
AB Background & aims. G-allele of PNPLA3 (rs738409) favours triglycerides accumulation and steatosis. In this study, we examined the effect of quercetin and natural extracts from mushroom and artichoke on reducing lipid accumulation in hepatic cells. Material and methods. Huh7.5 cells were exposed to oleic acid (OA) and treated with quercetin and extracts to observe the lipid accumulation, the intracellular-TG concentration and the LD size. Sterol regulatory element binding proteins-1 (SREBP-1), peroxisome proliferator-activated receptor (PPAR alpha-gamma) and cholesterol acyltransferase (ACAT) gene expression levels were analysed. Results. Quercetin decreased the intracellular lipids, LD size and the levels of intracellular-TG through the down-regulation of SREBP-1c, PPAR gamma and ACAT1 increasing PPAR alpha. The natural-extracts suppressed OA-induced lipid accumulation and the intracellular-TG. They down-regulate the hepatic lipogenesis through SREBP-1c, besides the activation of lipolysis through the increasing of PPAR alpha expression. Conclusions. Quercetin and the aqueous extracts decrease intracellular lipid accumulation by down-regulation of lipogenesis and up-regulation of lipolysis.
PB Mexican assoc hepatology
SN 1665-2681
YR 2018
FD 2018-03-01
LK http://hdl.handle.net/10668/19380
UL http://hdl.handle.net/10668/19380
LA en
DS RISalud
RD Apr 8, 2025