RT Conference Proceedings
T1 Detection of possible variant forms of hemoglobin in HbA1c measurements by chromatography liquid ion exchange high resolution (HPLC)
A1 Mayor, M.
A1 Cortés, M.
A1 Castro-Vega, I.
A1 Peña, A.
A1 Enguix-Armada, A.
K1 Cromatografía liquida
K1 Intercambio iónico
K1 Química clínica
K1 Hemoglobina A glucosilada
AB Background: The glycosylated hemoglobin (HbA1c) is used to help monitor the degree of a diabetic’s hyperglycemia. Security and accuracy of the methods used in its detection are affected by variants forms of Hb or elevations in levels of Fetal Hb (HbF). These interference are the result of a change in the haemoglobin total net charge of the variant due of a substitution of one amino acid in the remaining amino terminal of the beta chain. International Standardization for HbA1c values (NGSP) not include interference assessment as part of the certification program. Therefore, the effect of each variant or the lifting of the HbF on HbA1c result should be examined in each sample depending on the detected variant and the method used for the detection of the same. The objectives were: to describe the possible variants of Hb and their interference in HbA1c measurement by our method, after the implementation of a computer program for their detection. To identify some variants detected by chromatography liquid ion exchange high resolution (HPLC) with DNA molecular sequencing.
PB De Gruyter
SN 1434-6621
YR 2014
FD 2014-05-30
LK http://hdl.handle.net/10668/2054
UL http://hdl.handle.net/10668/2054
LA en
NO Mayor M, Cortés M, Castro-Vega I, Peña A, Enguix-Armada A. Detection of possible variant forms of hemoglobin in HbA1c measurements by chromatography liquid ion exchange high resolution ( HPLC). Clin Chem Lab Med. 2014; 52(Suppl. 1):S1279
DS RISalud
RD Apr 11, 2025