RT Journal Article
T1 Loss of 5hmC identifies a new type of aberrant DNA hypermethylation in glioma.
A1 Fernandez, Agustin F
A1 Bayon, Gustavo F
A1 Sierra, Marta I
A1 Urdinguio, Rocio G
A1 Toraño, Estela G
A1 Garcia, Maria G
A1 Carella, Antonella
A1 Lopez, Virginia
A1 Santamarina, Pablo
A1 Perez, Raul F
A1 Belmonte, Thalia
A1 Tejedor, Juan Ramon
A1 Cobo, Isabel
A1 Menendez, Pablo
A1 Mangas, Cristina
A1 Ferrero, Cecilia
A1 Rodrigo, Luis
A1 Astudillo, Aurora
A1 Ortea, Ignacio
A1 Cueto Diaz, Sergio
A1 Rodriguez-Gonzalez, Pablo
A1 Garcia Alonso, J Ignacio
A1 Mollejo, Manuela
A1 Melendez, Barbara
A1 Dominguez, Gemma
A1 Bonilla, Felix
A1 Fraga, Mario F
K1 5-Methylcytosine
K1 Biomarkers, tumor
K1 Case-control studies
K1 CpG islands
K1 DNA methylation
AB Aberrant DNA hypermethylation is a hallmark of cancer although the underlying molecular mechanisms are still poorly understood. To study the possible role of 5-hydroxymethylcytosine (5hmC) in this process we analyzed the global and locus-specific genome-wide levels of 5hmC and 5-methylcytosine (5mC) in human primary samples from 12 non-tumoral brains and 53 gliomas. We found that the levels of 5hmC identified in non-tumoral samples were significantly reduced in gliomas. Strikingly, hypo-hydroxymethylation at 4627 (9.3%) CpG sites was associated with aberrant DNA hypermethylation and was strongly enriched in CpG island shores. The DNA regions containing these CpG sites were enriched in H3K4me2 and presented a different genuine chromatin signature to that characteristic of the genes classically aberrantly hypermethylated in cancer. As this 5mC gain is inversely correlated with loss of 5hmC and has not been identified with classical sodium bisulfite-based technologies, we conclude that our data identifies a novel 5hmC-dependent type of aberrant DNA hypermethylation in glioma.
PB Oxford University Press
YR 2018
FD 2018-05-29
LK http://hdl.handle.net/10668/12561
UL http://hdl.handle.net/10668/12561
LA en
NO Fernandez AF, Bayón GF, Sierra MI, Urdinguio RG, Toraño EG, García MG, et al. C Loss of 5hmC identifies a new type of aberrant DNA hypermethylation in glioma. Hum Mol Genet. 2018 Sep 1;27(17):3046-3059
DS RISalud
RD Apr 5, 2025