RT Journal Article
T1 Fluorescent Calixarene-Schiff as a Nanovehicle with Biomedical Purposes
A1 Antonio Lebron, Jose
A1 Lopez-Lopez, Manuel
A1 Luisa Moya, Maria
A1 Deasy, Mary
A1 Munoz-Wic, Ana
A1 Beatriz Garcia-Calderon, Clara
A1 Valle Rosado, Ivan
A1 Lopez-Cornejo, Pilar
A1 Bernal, Eva
A1 Jose Ostos, Francisco
K1 calixarenes
K1 ct-DNA
K1 denaturation
K1 compaction
K1 gene therapy
K1 nanocarriers
K1 Dna-binding
K1 Condensation
K1 Delivery
K1 Surface
K1 Nanoparticles
K1 Transfection
K1 Langmuir
K1 Ions
AB Gene therapy is a technique that is currently under expansion and development. Recent advances in genetic medicine have paved the way for a broader range of therapies and laid the groundwork for next-generation technologies. A terminally substituted difluorene-diester Schiff Base calix[4]arene has been studied in this work as possible nanovector to be used in gene therapy. Changes to luminescent behavior of the calixarene macrocycle are reported in the presence of ct-DNA. The calixarene macrocycle interacts with calf thymus DNA (ct-DNA), generating changes in its conformation. Partial double-strand denaturation is induced at low concentrations of the calixarene, resulting in compaction of the ct-DNA. However, interaction between calixarene molecules themselves takes place at high calixarene concentrations, favoring the decompaction of the polynucleotide. Based on cytotoxicity studies, the calixarene macrocycle investigated has the potential to be used as a nanovehicle and improve the therapeutic efficacy of pharmacological agents against tumors.
PB Mdpi
YR 2022
FD 2022-07-01
LK http://hdl.handle.net/10668/20940
UL http://hdl.handle.net/10668/20940
LA en
DS RISalud
RD Apr 8, 2025