RT Journal Article
T1 The role of polymerase chain reaction of high-risk human papilloma virus in the screening of high-grade squamous intraepithelial lesions in the anal mucosa of human immunodeficiency virus-positive males having sex with males.
A1 Hidalgo-Tenorio, Carmen
A1 Rivero-Rodriguez, Mar
A1 Gil-Anguita, Concepción
A1 Esquivias, Javier
A1 López-Castro, Rodrigo
A1 Ramírez-Taboada, Jessica
A1 López de Hierro, Mercedes
A1 López-Ruiz, Miguel A
A1 Martínez, R Javier
A1 Llaño, Juan P
K1 Humanos
K1 Papillomaviridae
K1 Masculino
K1 Infecciones por papillomavirus
K1 Citodiagnóstico
K1 Reacción en cadena de la polimerasa
K1 Seropositividad para VIH
K1 Homosexualidad masculina
K1 Neoplasias del ano
K1 Instituciones de atención ambulatoria
K1 Biopsia
K1 Genotipo
K1 Infecciones por VIH
K1 Membrana mucosa
K1 Curva ROC
K1 Análisis de regresión
K1 Riesgo
AB OBJECTIVESTo evaluate the advantages of cytology and PCR of high-risk human papilloma virus (PCR HR-HPV) infection in biopsy-derived diagnosis of high-grade squamous intraepithelial lesions (HSIL = AIN2/AIN3) in HIV-positive men having sex with men (MSM).METHODSThis is a single-centered study conducted between May 2010 and May 2014 in patients (n = 201, mean age 37 years) recruited from our outpatient clinic. Samples of anal canal mucosa were taken into liquid medium for PCR HPV analysis and for cytology. Anoscopy was performed for histology evaluation.RESULTSAnoscopy showed 33.8% were normal, 47.8% low-grade squamous intraepithelial lesions (LSIL), and 18.4% HSIL; 80.2% had HR-HPV. PCR of HR-HPV had greater sensitivity than did cytology (88.8% vs. 75.7%) in HSIL screening, with similar positive (PPV) and negative predictive value (NPV) of 20.3 vs. 22.9 and 89.7 vs. 88.1, respectively. Combining both tests increased the sensitivity and NPV of HSIL diagnosis to 100%. Correlation of cytology vs. histology was, generally, very low and PCR of HR-HPV vs. histology was non-existent (<0.2) or low (<0.4). Area under the receiver operating characteristics (AUROC) curve analysis of cytology and PCR HR-HPV for the diagnosis of HSIL was poor (<0.6). Multivariate regression analysis showed protective factors against HSIL were: viral suppression (OR: 0.312; 95%CI: 0.099-0.984), and/or syphilis infection (OR: 0.193; 95%CI: 0.045-0.827). HSIL risk was associated with HPV-68 genotype (OR: 20.1; 95%CI: 2.04-197.82).CONCLUSIONSWhen cytology and PCR HR-HPV findings are normal, the diagnosis of pre-malignant HSIL can be reliably ruled-out in HIV-positive patients. HPV suppression with treatment protects against the appearance of HSIL.
PB Public Library of Science
YR 2015
FD 2015-04-07
LK http://hdl.handle.net/10668/2059
UL http://hdl.handle.net/10668/2059
LA en
NO Hidalgo-Tenorio C, Rivero-Rodriguez M, Gil-Anguita C, Esquivias J, López-Castro R, Ramírez-Taboada J, et al. The role of polymerase chain reaction of high-risk human papilloma virus in the screening of high-grade squamous intraepithelial lesions in the anal mucosa of human immunodeficiency virus-positive males having sex with males. PLoS ONE. 2015; 10(4):e0123590
NO Journal Article;
DS RISalud
RD Apr 13, 2025