RT Journal Article
T1 Monocyte Phenotype and Polyfunctionality Are Associated With Elevated Soluble Inflammatory Markers, Cytomegalovirus Infection, and Functional and Cognitive Decline in Elderly Adults.
A1 de Pablo-Bernal, Rebeca Sara
A1 Cañizares, Julio
A1 Rosado, Isaac
A1 Galvá, María Isabel
A1 Alvarez-Ríos, Ana Isabel
A1 Carrillo-Vico, Antonio
A1 Ferrando-Martínez, Sara
A1 Muñoz-Fernández, María Ángeles
A1 Rafii-El-Idrissi Benhnia, Mohammed
A1 Pacheco, Yolanda María
A1 Ramos, Raquel
A1 Leal, Manuel
A1 Ruiz-Mateos, Ezequiel
K1 Aging
K1 CMV
K1 Cognitive
K1 Inflammation
K1 Mini-mental
K1 Monocyte function
AB Monocytes are mediators of the inflammatory response and include three subsets: classical, intermediate, and nonclassical. Little is known about the phenotypical and functional age-related changes in monocytes and their association with soluble inflammatory biomarkers, cytomegalovirus infection, and functional and mental decline. We assayed the activation ex vivo and the responsiveness to TLR2 and TLR4 agonists in vitro in the three subsets and assessed the intracellular production of IL1-alpha (α), IL1-beta (β), IL-6, IL-8, TNF-α, and IL-10 of elderly adults (median 83 [67-90] years old;n= 20) compared with young controls (median 35 [27-40] years old;n= 20). Ex vivo, the elderly adults showed a higher percentage of classical monocytes that expressed intracellular IL1-α (p= .001), IL1-β (p= .001), IL-6 (p= .002), and IL-8 (p= .007). Similar results were obtained both for the intermediate and nonclassical subsets and in vitro. Polyfunctionality was higher in the elderly adults. The functionality ex vivo was strongly associated with soluble inflammatory markers. The activation phenotype was independently associated with the anti-cytomegalovirus IgG levels and with functional and cognitive decline. These data demonstrate that monocytes are key cell candidates for the source of the high soluble inflammatory levels. Our findings suggest that cytomegalovirus infection might be a driving force in the activation of monocytes and is associated with the functional and cognitive decline.
YR 2015
FD 2015-08-18
LK http://hdl.handle.net/10668/10094
UL http://hdl.handle.net/10668/10094
LA en
DS RISalud
RD Apr 7, 2025