RT Journal Article
T1 Munc18c in adipose tissue is downregulated in obesity and is associated with insulin.
A1 Garrido-Sánchez, Lourdes
A1 Escote, Xavier
A1 Coín-Aragüez, Leticia
A1 Fernández-García, José Carlos
A1 El Bekay, Rajaa
A1 Vendrell, Joan
A1 García-Fuentes, Eduardo
A1 Tinahones, Francisco J
K1 Resistencia a la Insulina
K1 Obesidad Mórbida
K1 Tejido Adiposo
K1 Glucosa
K1 HDL-Colesterol
K1 Regulación de la Expresión Génica
K1 Proteínas Munc18
AB OBJECTIVEMunc18c is associated with glucose metabolism and could play a relevant role in obesity. However, little is known about the regulation of Munc18c expression. We analyzed Munc18c gene expression in human visceral (VAT) and subcutaneous (SAT) adipose tissue and its relationship with obesity and insulin.MATERIALS AND METHODSWe evaluated 70 subjects distributed in 12 non-obese lean subjects, 23 overweight subjects, 12 obese subjects and 23 nondiabetic morbidly obese patients (11 with low insulin resistance and 12 with high insulin resistance).RESULTSThe lean, overweight and obese persons had a greater Munc18c gene expression in adipose tissue than the morbidly obese patients (p<0.001). VAT Munc18c gene expression was predicted by the body mass index (B = -0.001, p = 0.009). In SAT, no associations were found by different multiple regression analysis models. SAT Munc18c gene expression was the main determinant of the improvement in the HOMA-IR index 15 days after bariatric surgery (B = -2148.4, p = 0.038). SAT explant cultures showed that insulin produced a significant down-regulation of Munc18c gene expression (p = 0.048). This decrease was also obtained when explants were incubated with liver X receptor alpha (LXRα) agonist, either without (p = 0.038) or with insulin (p = 0.050). However, Munc18c gene expression was not affected when explants were incubated with insulin plus a sterol regulatory element-binding protein-1c (SREBP-1c) inhibitor (p = 0.504).CONCLUSIONSMunc18c gene expression in human adipose tissue is down-regulated in morbid obesity. Insulin may have an effect on the Munc18c expression, probably through LXRα and SREBP-1c.
PB Public Library of Science
YR 2013
FD 2013-05-20
LK http://hdl.handle.net/10668/1156
UL http://hdl.handle.net/10668/1156
LA en
NO Garrido-Sanchez L, Escote X, Coin-Aragüez L, Fernandez-Garcia JC, El Bekay R, Vendrell J, et al. Munc18c in adipose tissue is downregulated in obesity and is associated with insulin. PLoS ONE. 2013; 8(5):e63937
NO Journal Article;
DS RISalud
RD Apr 18, 2025