RT Journal Article
T1 Effects of Growth Medium and Inoculum Size on Pharmacodynamics Activity of Marbofloxacin against Staphylococcus aureus Isolated from Caprine Clinical Mastitis
A1 Lorenzutti, Augusto Matias
A1 San Andres-Larrea, Manuel Ignacio
A1 Fernandez-Varon, Emilio
A1 Zarazaga, Maria del Pilar
A1 Molina-Lopez, Ana Maria
A1 Serrano-Rodriguez, Juan Manuel
K1 marbofloxacin
K1 S. aureus
K1 inoculum size
K1 lactating goats
K1 clinical mastitis
K1 non-linear mixed effect modeling
K1 pharmacodynamics
K1 In-vitro
K1 Pseudomonas-aeruginosa
K1 Escherichia-coli
K1 Antimicrobial susceptibility
K1 Antibacterial activity
K1 Bactericidal activity
K1 Bovine mastitis
K1 Fluoroquinolone
K1 Ciprofloxacin
K1 Resistance
AB Staphylococcus aureus (S. aureus) is an important pathogen that causes clinical mastitis in goats and produces infections difficult to cure. Different antimicrobials as fluoroquinolones have been used against S. aureus. However, the studies developed to evaluate the bacterial drug interaction only have used the MIC as a single reference point with artificial growth media. The aims of this study were to describe the effect of marbofloxacin on S. aureus isolated from mastitis goats' milk by different approaches as the minimum inhibitory and bactericidal concentrations (MIC and MBC) in cation adjusted Mueller-Hinton broth (CAMHB), serum and milk of goats at two inoculum sizes of 10(5) and 10(8) CFU/mL, the determination and analysis of the time kill curves (TKC) by non-linear mixed effect models in each growth medium and inoculum size, as well as the estimation of their pharmacokinetics/pharmacodynamics (PK/PD) cutoff values. The results obtained indicate that MIC values were higher and increases 2,4-fold in serum and 3,6-fold in milk at high inoculum, as well as the EC50 values determined by each pharmacodynamics model. Finally, the PK/PD cutoff values defined as fAUC(24)/MIC ratios to achieve clinical efficacy were highly dependent on inoculum and growth medium, with median values of 60-180, especially at high inoculum in milk, suggesting that further studies are necessary to evaluate and optimize the best therapeutic strategies for treating S. aureus in lactating goats.
PB Mdpi
SN 2079-6382
YR 2021
FD 2021-11-01
LK https://hdl.handle.net/10668/28023
UL https://hdl.handle.net/10668/28023
LA en
DS RISalud
RD Apr 6, 2025