RT Journal Article
T1 Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii.
A1 Labrador-Herrera, Gema
A1 Perez-Pulido, Antonio J
A1 Alvarez-Marin, Rocio
A1 Casimiro-Soriguer, Carlos S
A1 Cebrero-Cangueiro, Tania
A1 Moran-Barrio, Jorgelina
A1 Pachon, Jeronimo
A1 Viale, Alejandro M
A1 Pachon-Ibañez, Maria Eugenia
K1 Acinetobacter baumannii
K1 CarO
K1 bloodstream infections
K1 carbapenem-resistance
K1 virulence factor
K1 whole-genome sequencing
AB Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections.
PB Landes Bioscience
YR 2020
FD 2020-12
LK http://hdl.handle.net/10668/16767
UL http://hdl.handle.net/10668/16767
LA en
NO Labrador-Herrera G, Pérez-Pulido AJ, Álvarez-Marín R, Casimiro-Soriguer CS, Cebrero-Cangueiro T, Morán-Barrio J, et al. Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii. Virulence. 2020 Dec;11(1):1727-1737.
DS RISalud
RD Oct 3, 2025